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Crystal Structure of the Human Rad9-Hus1-Rad1 Clamp SCIE SCOPUS

Title
Crystal Structure of the Human Rad9-Hus1-Rad1 Clamp
Authors
Sohn, SYCho, Y
Date Issued
2009-07-17
Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
Abstract
Three evolutionarily conserved proteins, Rad9, Hus1, and Rad1, form a heterotrimeric 9-1-1 complex that plays critical roles in cellular responses to DNA damage by activating checkpoints and by recruiting DNA repair enzymes to DNA lesions. We have determined the crystal structure of the human Rad9 (residues 1-272)-Hus1-Rad1 complex at 2.5 angstrom resolution. The 9(1-272)-1-1 complex forms a closed ring, with each subunit having a similar structure. Despite its high level of similarity to proliferating cell nucleus antigen in terms of overall structure, the 9(1-272)-1-1 complex exhibits notable differences in local structures, including interdomain connecting loops, H2 and H3 helices, and loops in the vicinity of the helices of each subunit. These local structural variations provide several unique features to the 9-1-1 heterotrimeric complex-including structures of intermolecular interfaces and the inner surface around the central hole, and different electrostatic potentials at and near the interdomain connecting loops of each 9-1-1 subunit-compared to the proliferating cell nucleus antigen trimer. We propose that these structural features allow the 9-1-1 complex to bind to a damaged DNA during checkpoint control and to serve as a platform for base excision repair. We also show that the 9(1-272)-1-1 complex, but not the full-length 9-1-1 complex, forms a stable complex with the 5' recessed DNA, suggesting that the C-terminal tail of Rad9 is involved in the regulation of the 9-1-1 complex in DNA binding. (C) 2009 Elsevier Ltd. All rights reserved.
Keywords
Rad9; Rad1; Hus1; DNA sliding clamp; checkpoint activation; DNA-DAMAGE CHECKPOINT; BASE EXCISION-REPAIR; SENSOR RAD9-RAD1-HUS1 INTERACTS; REPLICATION FACTOR-C; SCHIZOSACCHAROMYCES-POMBE; FISSION YEAST; FLAP ENDONUCLEASE-1; GENOMIC INSTABILITY; MOLECULAR-CLONING; PROTEIN COMPLEX
URI
https://oasis.postech.ac.kr/handle/2014.oak/26139
DOI
10.1016/J.JMB.2009.05.028
ISSN
0022-2836
Article Type
Article
Citation
JOURNAL OF MOLECULAR BIOLOGY, vol. 390, no. 3, page. 490 - 502, 2009-07-17
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조윤제CHO, YUNJE
Dept of Life Sciences
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