DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, GW | - |
dc.contributor.author | Cho, YS | - |
dc.contributor.author | Hwang, HS | - |
dc.contributor.author | Oh, MH | - |
dc.contributor.author | Nam, HG | - |
dc.contributor.author | Park, JH | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2016-04-01T03:06:53Z | - |
dc.date.available | 2016-04-01T03:06:53Z | - |
dc.date.created | 2010-04-20 | - |
dc.date.issued | 2009-08 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.other | 2009-OAK-0000020705 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26271 | - |
dc.description.abstract | Rapid diagnosis of bacterial infection is important for patient management and appropriate therapy during the early phase of bacteria-induced disease. Among the existing techniques for identifying microbial, CE-SSCP combined with 16S ribosomal RNA gene-specific PCR has the benefits of excellent sensitivity, resolution, and reproducibility. However, even though CE-SSCP can separate PCR products with high-resolution, multiplex detection and quantification are complicated by primer-dimer formation and non-specific amplification. Here, we describe a novel technique for multiplex detection and quantification of pathogens by template-tagging followed by multiplex asymmetric PCR and subsequent CE-SSCP. More specifically, we reverse transcribed 16S ribosomal RNAs from seven septicemia-inducing pathogens, tagged the templates with common end sequences, and amplified them using common primers. The resulting amplicons could be successfully separated by CE-SSCP and quantified by comparison to an internal standard. This method yielded results that illustrate the potential of this system for diagnosing infectious disease. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.relation.isPartOf | ELECTROPHORESIS | - |
dc.subject | CE-SSCP | - |
dc.subject | Common primer | - |
dc.subject | Diagnosis | - |
dc.subject | Pathogen | - |
dc.subject | Template-tagging | - |
dc.subject | RIBOSOMAL-RNA GENE | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | DNA MICROARRAY | - |
dc.subject | MICROBIAL COMMUNITY | - |
dc.subject | BACTERIAL PATHOGENS | - |
dc.subject | IDENTIFICATION | - |
dc.subject | ASSAY | - |
dc.subject | AMPLIFICATION | - |
dc.subject | DIAGNOSTICS | - |
dc.subject | SAMPLES | - |
dc.title | A new single-step quantitative pathogen detection system: Template-tagging followed by multiplex asymmetric PCR using common primers and CE-SSCP | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1002/ELPS.200900074 | - |
dc.author.google | Shin, GW | - |
dc.author.google | Cho, YS | - |
dc.author.google | Hwang, HS | - |
dc.author.google | Oh, MH | - |
dc.author.google | Nam, HG | - |
dc.author.google | Park, JH | - |
dc.author.google | Jung, GY | - |
dc.relation.volume | 30 | - |
dc.relation.issue | 15 | - |
dc.relation.startpage | 2728 | - |
dc.relation.lastpage | 2736 | - |
dc.contributor.id | 10130678 | - |
dc.relation.journal | ELECTROPHORESIS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ELECTROPHORESIS, v.30, no.15, pp.2728 - 2736 | - |
dc.identifier.wosid | 000269398700017 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 2736 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 2728 | - |
dc.citation.title | ELECTROPHORESIS | - |
dc.citation.volume | 30 | - |
dc.contributor.affiliatedAuthor | Nam, HG | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-70350786628 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 16 | - |
dc.description.scptc | 8 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | MICROBIAL COMMUNITY | - |
dc.subject.keywordPlus | BACTERIAL PATHOGENS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | MICROARRAYS | - |
dc.subject.keywordAuthor | CE-SSCP | - |
dc.subject.keywordAuthor | Common primer | - |
dc.subject.keywordAuthor | Diagnosis | - |
dc.subject.keywordAuthor | Pathogen | - |
dc.subject.keywordAuthor | Template-tagging | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.