DC Field | Value | Language |
---|---|---|
dc.contributor.author | Min, K | - |
dc.contributor.author | Cho, M | - |
dc.contributor.author | Han, SY | - |
dc.contributor.author | Shim, YB | - |
dc.contributor.author | Ku, J | - |
dc.contributor.author | Ban, C | - |
dc.date.accessioned | 2016-04-01T03:18:10Z | - |
dc.date.available | 2016-04-01T03:18:10Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2008-07-15 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.other | 2008-OAK-0000020348 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26486 | - |
dc.description.abstract | Tuberculosis is the most frequent cause of infection-related death worldwide. We constructed a simple and direct electrochemical sensor to detect interferon (IFN)-gamma, a selective marker for tuberculosis pleurisy, using its RNA and DNA aptamers. IFN-gamma was detected by its 5'-thiol-modified aptamer probe immobilized on the gold electrode. Interaction between IFN-gamma and the aptamer was recorded using electrochemical impedance spectroscopy and quartz crystal microbalance (QCM) with high sensitivity. The RNA-aptamer-based sensor showed a low detection limit of 100 fM, and the DNA-aptamer-based sensor detected IFN-gamma to 1 pM in sodium phosphate buffer. With QCM analysis, the aptamer immobilized on the electrode and IFN-gamma bound to the aptamer probe was quantified. This QCM result shows that IFN-gamma exists in multimeric forms to interact with the aptamers, and the RNA aptamer prefers the high multimeric state of IFN-gamma. Such a preference may describe the low detection limit of the RNA aptamer shown by impedance analysis. In addition, IFN-gamma was detected to 10 pM by the DNA aptamer in fetal bovine serum, a mimicked biological system, which has similar components to pleural fluid. (c) 2008 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.title | A simple and direct electrochemical detection of interferon-gamma using its RNA and DNA aptamers | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1016/j.bios.2008.02.021 | - |
dc.author.google | Min, K | - |
dc.author.google | Cho, M | - |
dc.author.google | Han, SY | - |
dc.author.google | Shim, YB | - |
dc.author.google | Ku, J | - |
dc.author.google | Ban, C | - |
dc.relation.volume | 23 | - |
dc.relation.issue | 12 | - |
dc.relation.startpage | 1819 | - |
dc.relation.lastpage | 1824 | - |
dc.relation.journal | BIOSENSORS & BIOELECTRONICS | - |
dc.relation.index | 국제일반학술지 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.23, no.12, pp.1819 - 1824 | - |
dc.identifier.wosid | 000256736300010 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1824 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1819 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 23 | - |
dc.contributor.affiliatedAuthor | Min, K | - |
dc.contributor.affiliatedAuthor | Ku, J | - |
dc.contributor.affiliatedAuthor | Ban, C | - |
dc.identifier.scopusid | 2-s2.0-43649089022 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 75 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TUBERCULOUS PLEURAL EFFUSIONS | - |
dc.subject.keywordPlus | IMPEDANCE SPECTROSCOPY | - |
dc.subject.keywordPlus | FLUORESCENCE ANISOTROPY | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | FLUID | - |
dc.subject.keywordPlus | OLIGODEOXYNUCLEOTIDES | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordAuthor | tuberculosis | - |
dc.subject.keywordAuthor | interferon-gamma (IFN-gamma) | - |
dc.subject.keywordAuthor | electrochemical impedance spectroscopy | - |
dc.subject.keywordAuthor | RNA aptamer | - |
dc.subject.keywordAuthor | DNA aptamer | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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