DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, JS | - |
dc.contributor.author | Park, IK | - |
dc.contributor.author | Cho, AS | - |
dc.contributor.author | Shin, SM | - |
dc.contributor.author | Hong, MH | - |
dc.contributor.author | Jeong, SY | - |
dc.contributor.author | Kim, YS | - |
dc.contributor.author | Min, JJ | - |
dc.contributor.author | Jeong, MH | - |
dc.contributor.author | Kim, WJ | - |
dc.contributor.author | Jo, S | - |
dc.contributor.author | Pun, SH | - |
dc.contributor.author | Cho, JG | - |
dc.contributor.author | Park, JC | - |
dc.contributor.author | Kang, JC | - |
dc.contributor.author | Ahn, Y | - |
dc.date.accessioned | 2016-04-01T03:21:51Z | - |
dc.date.available | 2016-04-01T03:21:51Z | - |
dc.date.created | 2010-03-31 | - |
dc.date.issued | 2009-09-01 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.other | 2009-OAK-0000020219 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26532 | - |
dc.description.abstract | Thermo-responsive hydrogel-mediated gene transfer may be preferred for the muscle, because the release of DNA into the surrounding tissue can be controlled by the 3-dimensional structure of the hydrogel. Such a system for the controlled release of a therapeutic gene may extend the duration of gene expression. Here, a thermo-responsive, biodegradable polymeric hydrogel was synthesized for local gene transfer in the heart. Initially, the luciferase gene was delivered into mouse heart. The intensity of gene expression assessed by optical imaging was closely correlated with the expressed protein concentration measured by luciferase assay in homogenized heart. Polymeric hydrogel-based gene transfer enhanced gene expression up to 4 fold, compared with naked plasmid, and displayed 2 bi-modal expression profiles with peaks at 2 days and around 25 days after local injection. Histological analyses showed that gene expression was initially highest in the myocardium, whereas lower and longer expression was seen mainly in fibrotic or inflammatory cells that infiltrated the injury site during injection. Next, a rat myocardial infarction model was made for 1 week, and human vascular endothelial growth factor (hVEGF) plasmid was injected into the infarct area with an amphiphilic thermo-responsive polymer. Enhanced and sustained hVEGF expression in the infarct region mediated by amphiphilic thermo-responsive polymer increased capillary density and larger vessel formation, thus enabling effective angiogenesis. (C) 2009 Elsevier B.V. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.subject | Temperature-responsive amphiphilic polymer | - |
dc.subject | Local gene transfer | - |
dc.subject | Angiogenesis | - |
dc.subject | Myocardial infarction | - |
dc.subject | INTRAMUSCULAR GENE-TRANSFER | - |
dc.subject | MOUSE SKELETAL-MUSCLE | - |
dc.subject | IN-VIVO | - |
dc.subject | EXTRACELLULAR GLYCOSAMINOGLYCANS | - |
dc.subject | DEOXYRIBONUCLEIC-ACID | - |
dc.subject | ADENOVIRUS VECTORS | - |
dc.subject | DELIVERY-SYSTEMS | - |
dc.subject | PORCINE HEART | - |
dc.subject | THERAPY | - |
dc.subject | EXPRESSION | - |
dc.title | Enhanced angiogenesis mediated by vascular endothelial growth factor plasmid-loaded thermo-responsive amphiphilic polymer in a rat myocardial infarction model | - |
dc.type | Article | - |
dc.contributor.college | 화학과 | - |
dc.identifier.doi | 10.1016/J.JCONREL.2009.05.023 | - |
dc.author.google | Kwon, JS | - |
dc.author.google | Park, IK | - |
dc.author.google | Cho, AS | - |
dc.author.google | Shin, SM | - |
dc.author.google | Hong, MH | - |
dc.author.google | Jeong, SY | - |
dc.author.google | Kim, YS | - |
dc.author.google | Min, JJ | - |
dc.author.google | Jeong, MH | - |
dc.author.google | Kim, WJ | - |
dc.author.google | Jo, S | - |
dc.author.google | Pun, SH | - |
dc.author.google | Cho, JG | - |
dc.author.google | Park, JC | - |
dc.author.google | Kang, JC | - |
dc.author.google | Ahn, Y | - |
dc.relation.volume | 138 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 168 | - |
dc.relation.lastpage | 176 | - |
dc.contributor.id | 10135304 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.138, no.2, pp.168 - 176 | - |
dc.identifier.wosid | 000270926700011 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 176 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 168 | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 138 | - |
dc.contributor.affiliatedAuthor | Kim, WJ | - |
dc.identifier.scopusid | 2-s2.0-67849124168 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 18 | - |
dc.description.scptc | 25 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INTRAMUSCULAR GENE-TRANSFER | - |
dc.subject.keywordPlus | MOUSE SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | EXTRACELLULAR GLYCOSAMINOGLYCANS | - |
dc.subject.keywordPlus | DEOXYRIBONUCLEIC-ACID | - |
dc.subject.keywordPlus | ADENOVIRUS VECTORS | - |
dc.subject.keywordPlus | DELIVERY-SYSTEMS | - |
dc.subject.keywordPlus | PORCINE HEART | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | Temperature-responsive amphiphilic polymer | - |
dc.subject.keywordAuthor | Local gene transfer | - |
dc.subject.keywordAuthor | Angiogenesis | - |
dc.subject.keywordAuthor | Myocardial infarction | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.