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Cited 114 time in webofscience Cited 128 time in scopus
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Molecular signature of late-stage human ALS revealed by expression profiling of postmortem spinal cord gray matter SCIE SCOPUS

Title
Molecular signature of late-stage human ALS revealed by expression profiling of postmortem spinal cord gray matter
Authors
Dangond, FHwang, DCamelo, SPasinelli, PFrosch, MPStephanopoulos, GStephanopoulos, GBrown, RHGullans, SR
Date Issued
2004-01-15
Publisher
AMER PHYSIOLOGICAL SOC
Abstract
Little is known about global gene expression patterns in the human neurodegenerative disease amyotrophic lateral sclerosis (ALS). To address this, we used high-density oligonucleotide microarray technology to compare expression levels of similar to6,800 genes in postmortem spinal cord gray matter obtained from individuals with ALS as well as normal individuals. Using Fisher discriminant analysis (FDA) and leave-one-out cross-validation (LOOCV), we discerned an ALS-specific signature. Moreover, it was possible to distinguish familial ALS (FALS) from sporadic ALS (SALS) gene expression profiles. Characterization of the specific genes significantly altered in ALS uncovered a pro-inflammatory terminal state. Moreover, we found alterations in genes involved in mitochondrial function, oxidative stress, excitotoxicity, apoptosis, cytoskeletal architecture, RNA transcription and translation, proteasomal function, and growth and signaling. It is apparent from this study that DNA microarray analysis and appropriate bioinformatics can reveal distinct phenotypic changes that underlie the terminal stages of neurodegeneration in ALS.
Keywords
amyotrophic lateral sclerosis; DNA microarrays; mitochondria; excitotoxicity; apoptosis; TRANSGENIC MOUSE MODEL; MOTOR-NEURON DISEASE; SUPEROXIDE-DISMUTASE; NEURODEGENERATIVE DISEASE; DIFFERENTIAL EXPRESSION; GLUTAMATE TRANSPORTER; OXIDATIVE STRESS; CDNA MICROARRAYS; GENE-EXPRESSION; SCLEROSIS
URI
https://oasis.postech.ac.kr/handle/2014.oak/26541
DOI
10.1152/PHYSIOLGENOMICS.00087.2001
ISSN
1094-8341
Article Type
Article
Citation
PHYSIOLOGICAL GENOMICS, vol. 16, no. 2, page. 229 - 239, 2004-01-15
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황대희HWANG, DAEHEE
Div of Integrative Biosci & Biotech
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