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Cited 23 time in webofscience Cited 22 time in scopus
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Nanoengineered Polystyrene Surfaces with Nanopore Array Pattern Alters Cytoskeleton Organization and Enhances Induction of Neural Differentiation of Human Adipose-Derived Stem Cells SCIE SCOPUS

Title
Nanoengineered Polystyrene Surfaces with Nanopore Array Pattern Alters Cytoskeleton Organization and Enhances Induction of Neural Differentiation of Human Adipose-Derived Stem Cells
Authors
Ae Ryang JungRichard Y. KimHyung Woo KimKshitiz Raj ShresthaSeung Hwan JeonKyoung Je ChaYong Hyun ParkKim, DSJi Youl Lee
Date Issued
2015-07
Publisher
MARY ANN LIEBERT, INC
Abstract
Human adipose-derived stem cells (hADSCs) can differentiate into various cell types depending on chemical and topographical cues. One topographical cue recently noted to be successful in inducing differentiation is the nanoengineered polystyrene surface containing nanopore array-patterned substrate (NP substrate), which is designed to mimic the nanoscale topographical features of the extracellular matrix. In this study, efficacies of NP and flat substrates in inducing neural differentiation of hADSCs were examined by comparing their substrate-cell adhesion rates, filopodia growth, nuclei elongation, and expression of neural-specific markers. The polystyrene nano Petri dishes containing NP substrates were fabricated by a nano injection molding process using a nickel electroformed nano-mold insert (Diameter: 200nm. Depth of pore: 500nm. Center-to-center distance: 500nm). Cytoskeleton and filopodia structures were observed by scanning electron microscopy and F-actin staining, while cell adhesion was tested by vinculin staining after 24 and 48h of seeding. Expression of neural specific markers was examined by real-time quantitative polymerase chain reaction and immunocytochemistry. Results showed that NP substrates lead to greater substrate-cell adhesion, filopodia growth, nuclei elongation, and expression of neural specific markers compared to flat substrates. These results not only show the advantages of NP substrates, but they also suggest that further study into cell-substrate interactions may yield great benefits for biomaterial engineering.
URI
https://oasis.postech.ac.kr/handle/2014.oak/26650
DOI
10.1089/TEN.TEA.2014.0346
ISSN
1937-3341
Article Type
Article
Citation
TISSUE ENGINEERING PART A, vol. 21, no. 13, page. 2115 - 2124, 2015-07
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김동성KIM, DONG SUNG
Dept of Mechanical Enginrg
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