Nanoengineered Polystyrene Surfaces with Nanopore Array Pattern Alters Cytoskeleton Organization and Enhances Induction of Neural Differentiation of Human Adipose-Derived Stem Cells
SCIE
SCOPUS
- Title
- Nanoengineered Polystyrene Surfaces with Nanopore Array Pattern Alters Cytoskeleton Organization and Enhances Induction of Neural Differentiation of Human Adipose-Derived Stem Cells
- Authors
- Ae Ryang Jung; Richard Y. Kim; Hyung Woo Kim; Kshitiz Raj Shrestha; Seung Hwan Jeon; Kyoung Je Cha; Yong Hyun Park; Kim, DS; Ji Youl Lee
- Date Issued
- 2015-07
- Publisher
- MARY ANN LIEBERT, INC
- Abstract
- Human adipose-derived stem cells (hADSCs) can differentiate into various cell types depending on chemical and topographical cues. One topographical cue recently noted to be successful in inducing differentiation is the nanoengineered polystyrene surface containing nanopore array-patterned substrate (NP substrate), which is designed to mimic the nanoscale topographical features of the extracellular matrix. In this study, efficacies of NP and flat substrates in inducing neural differentiation of hADSCs were examined by comparing their substrate-cell adhesion rates, filopodia growth, nuclei elongation, and expression of neural-specific markers. The polystyrene nano Petri dishes containing NP substrates were fabricated by a nano injection molding process using a nickel electroformed nano-mold insert (Diameter: 200nm. Depth of pore: 500nm. Center-to-center distance: 500nm). Cytoskeleton and filopodia structures were observed by scanning electron microscopy and F-actin staining, while cell adhesion was tested by vinculin staining after 24 and 48h of seeding. Expression of neural specific markers was examined by real-time quantitative polymerase chain reaction and immunocytochemistry. Results showed that NP substrates lead to greater substrate-cell adhesion, filopodia growth, nuclei elongation, and expression of neural specific markers compared to flat substrates. These results not only show the advantages of NP substrates, but they also suggest that further study into cell-substrate interactions may yield great benefits for biomaterial engineering.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26650
- DOI
- 10.1089/TEN.TEA.2014.0346
- ISSN
- 1937-3341
- Article Type
- Article
- Citation
- TISSUE ENGINEERING PART A, vol. 21, no. 13, page. 2115 - 2124, 2015-07
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.