DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shim, SY | - |
dc.contributor.author | de Castro, IP | - |
dc.contributor.author | Neumayer, G | - |
dc.contributor.author | Wang, J | - |
dc.contributor.author | Park, SK | - |
dc.contributor.author | Sanada, K | - |
dc.contributor.author | Nguyen, MD | - |
dc.date.accessioned | 2016-04-01T07:47:39Z | - |
dc.date.available | 2016-04-01T07:47:39Z | - |
dc.date.created | 2015-06-22 | - |
dc.date.issued | 2015-04-03 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2015-OAK-0000033100 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/26879 | - |
dc.description.abstract | The human ortholog of the targeting protein for Xenopus kinesin-like protein 2 (TPX2) is a cytoskeletal protein that plays a major role in spindle assembly and is required for mitosis. During spindle morphogenesis, TPX2 cooperates with Aurora A kinase and Eg5 kinesin to regulate microtubule organization. TPX2 displays over 40 putative phosphorylation sites identified from various high-throughput proteomic screenings. In this study, we characterize the phosphorylation of threonine 72 (Thr(72)) in human TPX2, a residue highly conserved across species. We find that Cdk1/2 phosphorylate TPX2 in vitro and in vivo. Using homemade antibodies specific for TPX2 phosphorylated at Thr(72), we show that this phosphorylation is cell cycle-dependent and peaks at M phase. Endogenous TPX2 phosphorylated at Thr(72) does not associate with the mitotic spindle. Furthermore, ectopic GFP-TPX2 T72A preferentially concentrates on the spindle, whereas GFP-TPX2 WT distributes to both spindle and cytosol. The T72A mutant also increases the proportion of cells with multipolar spindles phenotype. This effect is associated with increased Aurora A activity and abnormally elongated spindles, indicative of higher Eg5 activity. In summary, we propose that phosphorylation of Thr(72) regulates TPX2 localization and impacts spindle assembly via Aurora A and Eg5. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.relation.isPartOf | Journal of Biological Chemistry | - |
dc.title | Phosphorylation of Targeting Protein for Xenopus Kinesin-like Protein 2 (TPX2) at Threonine 72 in Spindle Assembly | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/JBC.M114.591545 | - |
dc.author.google | Shim, SY | - |
dc.author.google | de Castro, IP | - |
dc.author.google | Neumayer, G | - |
dc.author.google | Wang, J | - |
dc.author.google | Park, SK | - |
dc.author.google | Sanada, K | - |
dc.author.google | Nguyen, MD | - |
dc.relation.volume | 290 | - |
dc.relation.issue | 14 | - |
dc.relation.startpage | 9122 | - |
dc.relation.lastpage | 9134 | - |
dc.contributor.id | 10149637 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Journal of Biological Chemistry, v.290, no.14, pp.9122 - 9134 | - |
dc.identifier.wosid | 000352207100035 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 9134 | - |
dc.citation.number | 14 | - |
dc.citation.startPage | 9122 | - |
dc.citation.title | Journal of Biological Chemistry | - |
dc.citation.volume | 290 | - |
dc.contributor.affiliatedAuthor | Park, SK | - |
dc.identifier.scopusid | 2-s2.0-84926442197 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 10 | - |
dc.description.scptc | 9 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CYCLIN-DEPENDENT KINASES | - |
dc.subject.keywordPlus | AURORA-A ACTIVATION | - |
dc.subject.keywordPlus | QUANTITATIVE PHOSPHOPROTEOMICS | - |
dc.subject.keywordPlus | MICROTUBULE NUCLEATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | CDK2 | - |
dc.subject.keywordPlus | CDC2 | - |
dc.subject.keywordPlus | PHOSPHOSITEPLUS | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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