Open Access System for Information Sharing

Login Library

 

Article
Cited 95 time in webofscience Cited 0 time in scopus
Metadata Downloads

A lysosomal tetraspanin associated with retinal degeneration identified via a genome-wide screen SCIE SCOPUS

Title
A lysosomal tetraspanin associated with retinal degeneration identified via a genome-wide screen
Authors
Xu, HLee, SJSuzuki, EDugan, KDStoddard, ALi, HSChodosh, LAMontell, C
Date Issued
2004-02-25
Publisher
European Molecular Biology Organization Press
Abstract
The Drosophila visual system has provided a model to study phototransduction and retinal degeneration. To identify new candidate proteins that contribute to these processes, we conducted a genome-wide screen for genes expressed predominately in the eye, using DNA microarrays. This screen appeared to be comprehensive as it led to the identification of all 22 eye-enriched genes previously shown to function in phototransduction or implicated in retinal degeneration. In addition, we identified 93 eye-enriched genes whose roles have not been previously defined. One of the eye-enriched genes encoded a member of a large family of transmembrane proteins, referred to as tetraspanins. We created a null mutation in the eye-enriched tetraspanin, Sunglasses (Sun), which resulted in light-induced retinal degeneration. We found that the Sun protein was distributed primarily in lysosomes, and functioned in a long-known but poorly understood phenomenon of light-induced degradation of rhodopsin. We propose that lysosomal tetraspanins in mammalian cells may also function in the downregulation of rhodopsin and other G-protein-coupled receptors, in response to intense or prolonged agonist stimulation.
Keywords
Drosophila; microarray; phototransduction; retinal degeneration; rhodopsin; tetraspanin; DROSOPHILA-MELANOGASTER; GENE-EXPRESSION; PHOTORECEPTOR CELLS; PROTEIN; ARRESTIN; COMPLEX; CDNA; INAD; TRP; TRANSLOCATION
URI
https://oasis.postech.ac.kr/handle/2014.oak/27586
DOI
10.1038/sj.emboj.7600112
ISSN
0261-4189
Article Type
Article
Citation
The EMBO Journal, vol. 23, no. 4, page. 811 - 822, 2004-02-25
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

이승재LEE, SEUNG JAE
Dept of Life Sciences
Read more

Views & Downloads

Browse