DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cha, HJ | - |
dc.contributor.author | Kim, KR | - |
dc.contributor.author | Hwang, BH | - |
dc.contributor.author | Ahn, DH | - |
dc.contributor.author | Yoo, YJ | - |
dc.date.accessioned | 2016-04-01T08:27:02Z | - |
dc.date.available | 2016-04-01T08:27:02Z | - |
dc.date.created | 2009-09-28 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.issn | 0256-1115 | - |
dc.identifier.other | 2007-OAK-0000019016 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28001 | - |
dc.description.abstract | Glucoamylase that hydrolyses starch to glucose is one of the important industrial enzymes for ethanol production industry. Therefore, genetic production of recombinant glucoamylase has been widely studied. Previously, we reported secreted production of Saccharomyces diastaticus-originated glucoamylase in Saccharomyces cerevisiase expression system using its own signal sequence and the SUC2 promoter that is regulated by glucose level in culture medium. In the present work, we performed a comparative study between batch and fed-batch bioreactor cultures for secreted production of recombinant glucoamylase. Through maintaining low glucose levels in the culture broth, we obtained about 7-fold higher secreted production levels of glucoamlyase in fed-batch culture. Fed-batch culture strategy also enhanced (similar to 3.1-fold) secretion efficiency of recombinant glucoamylase in S. cerevisiae. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | KOREAN INST CHEM ENGINEERS | - |
dc.relation.isPartOf | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.subject | recombinant yeast | - |
dc.subject | glucoamylase | - |
dc.subject | SUC2 promoter | - |
dc.subject | secreted production | - |
dc.subject | fed-batch culture | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | CLONED GLUCOAMYLASE | - |
dc.subject | INVERTASE | - |
dc.subject | EXPRESSION | - |
dc.subject | SEQUENCE | - |
dc.title | Enhancement of secreted production of glucoamylase through fed-batch bioreactor culture of recombinant yeast harboring glucose-controllable SUC2 promoter | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1007/s11814-007-0046-2 | - |
dc.author.google | Cha, HJ | - |
dc.author.google | Kim, KR | - |
dc.author.google | Hwang, BH | - |
dc.author.google | Ahn, DH | - |
dc.author.google | Yoo, YJ | - |
dc.relation.volume | 24 | - |
dc.relation.issue | 5 | - |
dc.relation.startpage | 812 | - |
dc.relation.lastpage | 815 | - |
dc.relation.journal | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCIE | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.24, no.5, pp.812 - 815 | - |
dc.identifier.wosid | 000250077100015 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 815 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 812 | - |
dc.citation.title | KOREAN JOURNAL OF CHEMICAL ENGINEERING | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.contributor.affiliatedAuthor | Kim, KR | - |
dc.identifier.scopusid | 2-s2.0-36849004965 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 4 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | CLONED GLUCOAMYLASE | - |
dc.subject.keywordPlus | INVERTASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordAuthor | recombinant yeast | - |
dc.subject.keywordAuthor | glucoamylase | - |
dc.subject.keywordAuthor | SUC2 promoter | - |
dc.subject.keywordAuthor | secreted production | - |
dc.subject.keywordAuthor | fed-batch culture | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
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