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EXPRESSION AND STABILITY OF RECOMBINANT RQ-MESSENGER-RNAS IN CELL-FREE TRANSLATION SYSTEMS SCIE SCOPUS
- Title
- EXPRESSION AND STABILITY OF RECOMBINANT RQ-MESSENGER-RNAS IN CELL-FREE TRANSLATION SYSTEMS
- Authors
- UGAROV, VI; MOROZOV, IY; JUNG, GY; CHETVERIN, AB; SPIRIN, AS
- Date Issued
- 1994-03-14
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.
- ISSN
- 0014-5793
- Article Type
- Article
- Citation
- FEBS LETTERS, vol. 341, no. 1, page. 131 - 134, 1994-03-14
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