DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, CH | - |
dc.contributor.author | Parashurama, N | - |
dc.contributor.author | Park, EYH | - |
dc.contributor.author | Suganuma, K | - |
dc.contributor.author | Nahniias, Y | - |
dc.contributor.author | Park, J | - |
dc.contributor.author | Tilles, AW | - |
dc.contributor.author | Berthiaume, F | - |
dc.contributor.author | Yarmush, ML | - |
dc.date.accessioned | 2016-04-01T08:32:16Z | - |
dc.date.available | 2016-04-01T08:32:16Z | - |
dc.date.created | 2009-09-03 | - |
dc.date.issued | 2008-03 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.other | 2008-OAK-0000018579 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28198 | - |
dc.description.abstract | One of the major hurdles of cellular therapies for the treatment of liver failure is the low availability of functional human hepatocytes. While embryonic stem (ES) cells represent a potential cell source for therapy, current methods for differentiation result in mixed cell populations or low yields of the cells of interest. Here we describe a rapid, direct differentiation method that yields a homogeneous population of endoderm-like cells with 95% purity. Mouse ES cells cultured on top of collagen-sandwiched hepatocytes differentiated and proliferated into a uniform and homogeneous cell population of endoderm-like cells. The endoderm-like cell population was positive for Foxa2, Sox17, and AFP and could be further differentiated into hepatocyte-like cells, demonstrating hepatic morphology, functionality, and gene and protein expression. Incorporating the hepatocyte-like cells into a bioartificial liver device to treat fulminant hepatic failure improved animal survival, thereby underscoring the therapeutic potential of these cells.-Cho, C. H., Parashurama, N., Park, E. Y. H., Sugamuna, K, Nahmias, Y., Park, J., Tilles, A. W., Berthiaume, F., Yarmush, M. L. Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | FEDERATION AMER SOC EXP BIOL | - |
dc.relation.isPartOf | FASEB JOURNAL | - |
dc.subject | coculture | - |
dc.subject | endoderm | - |
dc.subject | animal model | - |
dc.subject | bioartificial liver device | - |
dc.subject | IN-VITRO DIFFERENTIATION | - |
dc.subject | DEFINITIVE ENDODERM | - |
dc.subject | GROWTH-FACTOR | - |
dc.subject | HEPATIC DIFFERENTIATION | - |
dc.subject | SANDWICH CONFIGURATION | - |
dc.subject | BIOARTIFICIAL LIVER | - |
dc.subject | COLLAGEN SANDWICH | - |
dc.subject | ENDOTHELIAL-CELLS | - |
dc.subject | CONTROLLED-TRIAL | - |
dc.subject | GENE-EXPRESSION | - |
dc.title | Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure | - |
dc.type | Article | - |
dc.contributor.college | 기계공학과 | - |
dc.identifier.doi | 10.1096/FJ.06-7764COM | - |
dc.author.google | Cho, CH | - |
dc.author.google | Parashurama, N | - |
dc.author.google | Park, EYH | - |
dc.author.google | Suganuma, K | - |
dc.author.google | Nahniias, Y | - |
dc.author.google | Park, J | - |
dc.author.google | Tilles, AW | - |
dc.author.google | Berthiaume, F | - |
dc.author.google | Yarmush, ML | - |
dc.relation.volume | 22 | - |
dc.relation.issue | 3 | - |
dc.relation.startpage | 898 | - |
dc.relation.lastpage | 909 | - |
dc.contributor.id | 10093923 | - |
dc.relation.journal | FASEB JOURNAL | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | FASEB JOURNAL, v.22, no.3, pp.898 - 909 | - |
dc.identifier.wosid | 000254143700028 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 909 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 898 | - |
dc.citation.title | FASEB JOURNAL | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Park, J | - |
dc.identifier.scopusid | 2-s2.0-40449118677 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 50 | - |
dc.description.scptc | 52 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | IN-VITRO DIFFERENTIATION | - |
dc.subject.keywordPlus | DEFINITIVE ENDODERM | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | HEPATIC DIFFERENTIATION | - |
dc.subject.keywordPlus | SANDWICH CONFIGURATION | - |
dc.subject.keywordPlus | BIOARTIFICIAL LIVER | - |
dc.subject.keywordPlus | COLLAGEN SANDWICH | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | CONTROLLED-TRIAL | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordAuthor | coculture | - |
dc.subject.keywordAuthor | endoderm | - |
dc.subject.keywordAuthor | animal model | - |
dc.subject.keywordAuthor | bioartificial liver device | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Cell Biology | - |
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