TYPE II CAMP-DEPENDENT PROTEIN KINASE-DEFICIENT DROSOPHILA ARE VIABLE BUT SHOW DEVELOPMENTAL, CIRCADIAN, AND DRUG RESPONSE PHENOTYPES
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- Title
- TYPE II CAMP-DEPENDENT PROTEIN KINASE-DEFICIENT DROSOPHILA ARE VIABLE BUT SHOW DEVELOPMENTAL, CIRCADIAN, AND DRUG RESPONSE PHENOTYPES
- Authors
- Park, SK; Sedore, SA; Cronmiller, C; Hirsh, J
- Date Issued
- 2000-07-07
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLO
- Abstract
- We identified a unique type II cAMP-dependent protein kinase regulatory subunit (PKA-RII) gene in Drosophila melanogaster and a severely hypomorphic if not null mutation, pka-RIIEP(2)2162. Extracts from pka-RIIEP(2)2162 flies selectively lack RII-specific autophosphorylation activity and show significantly reduced cAMP binding activity, attributable to the loss of functional PKA-RII, pka-RIIEP(2)2162 shows 2-fold increased basal PKA activity and similar to 40% of normal cAMP-inducible PKA activity. pka-RIIEP(2)2162 is fully viable but displays abnormalities of ovarian development and multiple behavioral phenotypes including arrhythmic circadian locomotor activity, decreased sensitivity to ethanol and cocaine, and a lack of sensitization to repeated cocaine exposures. These findings implicate type II PKA activity in these processes in Drosophila and imply a common role for PKA signaling in regulating responsiveness to cocaine and alcohol.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28228
- DOI
- 10.1074/jbc.M002460200
- ISSN
- 0021-9258
- Article Type
- Article
- Citation
- JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 275, no. 27, page. 20588 - 20596, 2000-07-07
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