DC Field | Value | Language |
---|---|---|
dc.contributor.author | HAN, SE | - |
dc.contributor.author | KANG, H | - |
dc.contributor.author | SHIM, GY | - |
dc.contributor.author | KIM, SJ | - |
dc.contributor.author | CHOI, HG | - |
dc.contributor.author | KIM, J | - |
dc.contributor.author | HAHN, SK | - |
dc.contributor.author | OH, YK | - |
dc.contributor.author | null | - |
dc.date.accessioned | 2016-04-01T08:35:54Z | - |
dc.date.available | 2016-04-01T08:35:54Z | - |
dc.date.issued | 2009-02 | - |
dc.identifier.citation | JOURNAL OF DRUG TARGETING | - |
dc.identifier.citation | v.17 | - |
dc.identifier.citation | no.2 | - |
dc.identifier.citation | pp.123-132 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.other | 2009-OAK-0000018326 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28334 | - |
dc.description.abstract | In this study, we tested the use of cationic polymer derivatives of biocompatible hyaluronic acid (HA) as a delivery system of siRNA and antisense oligonucleotides. HA was modified with cationic polymer polyethylenimine (PEI). When compared with PEI alone, cationic PEI derivatives of HA (HA-PEI) provided increased cellular delivery of Small interfering RNA (siRNA) in B16F1, A549, HeLa, and Hep3B tumor cells. Indeed, more than 95% of the cells were positive for siRNA following its delivery with HA-PEI. A survivin-specific siRNA that was delivered using HA-PEI potently reduced the mRNA expression levels of the target gene in all of the cell lines. By contrast, survivin-specific siRNA delivered by PEI alone did not induce a significant reduction in mRNA levels. In green fluorescent protein (GFP)-expressing 293 T cells, a loss of GFP expression was evident in the cells that had been treated with GFP-specific siRNA and HA-PEI complex. The inhibition of target gene expression by antisense oligonucleotide 63139 was also enhanced after delivery with HA-PEI. Moreover, HA-PEI displayed lower cytotoxicity than PEI alone. These results suggest that HA-PEI could be further developed as biocompatible delivery systems of siRNA and antisense oligonucleotides for enhanced cellular uptake and inhibition of target gene expression. | - |
dc.description.statementofresponsibility | X | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.subject | Hyaluronic acid | - |
dc.subject | cationic polymer | - |
dc.subject | siRNA delivery | - |
dc.subject | antisense oligonucleotides | - |
dc.subject | PLASMID DNA | - |
dc.subject | CELLULAR UPTAKE | - |
dc.subject | IN-VIVO | - |
dc.subject | POLYETHYLENIMINE | - |
dc.subject | CELLS | - |
dc.subject | EXPRESSION | - |
dc.subject | COMPLEXES | - |
dc.subject | CANCER | - |
dc.title | Cationic derivatives of biocompatible hyaluronic acids for delivery of siRNA and antisense oligonucleotides | - |
dc.type | Article | - |
dc.identifier.doi | 10.1080/106118608024 | - |
dc.author.google | HAN, SE | - |
dc.author.google | KANG, H | - |
dc.author.google | SHIM, GY | - |
dc.author.google | KIM, SJ | - |
dc.author.google | CHOI, HG | - |
dc.author.google | KIM, J | - |
dc.author.google | HAHN, SK | - |
dc.author.google | OH, YK | - |
dc.relation.volume | 17 | - |
dc.relation.issue | 2 | - |
dc.relation.startpage | 123 | - |
dc.relation.lastpage | 132 | - |
dc.publisher.location | UK | - |
dc.relation.journal | JOURNAL OF DRUG TARGETING | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.docType | Article | - |
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