SYNTHETIC SURFACES AS ARTIFICIAL ANTIGEN PRESENTING CELLS IN THE STUDY OF T CELL RECEPTOR TRIGGERING AND IMMUNOLOGICAL SYNAPSE FORMATION

Abstract

T cell activation occurs when T cell receptors engage peptide-major histocompatibility complex (pMHC molecules displayed on the surface of antigen presenting cells (APCs). Clustering of TCRs and other receptors in physical patterns at the T-APC interface forms a structure known as an immunological synapse (IS). Studies of the IS are challenging due to the cell-cell contact context of the governing interactions. Model surfaces as synthetic APCs have thus been developed, where the type, quantity, and physical arrangement of ligands displayed to T cells are precisely controlled. These model systems have provided important insights into the structure and function of the IS. (c) 2007 Elsevier Ltd. All rights reserved.