Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production
SCIE
SCOPUS
- Title
- Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production
- Authors
- Kim, TD; Woo, KC; Cho, S; Ha, DC; Jang, SK; Kim, KT
- Date Issued
- 2007-04-01
- Publisher
- COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
- Abstract
- The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its species-specific mRNA profiles. Here we show that the circadian timing of AANAT protein expression is regulated by rhythmic translation of AANAT mRNA. This rhythmic control is mediated by both a highly conserved IRES (internal ribosome entry site) element within the AANAT 5' untranslated region and its partner hnRNP Q (heterogeneous nuclear ribonucleoprotein Q) with a peak in the middle of the night. Consistent with the enhancing role of hnRNP Q in AANAT IRES activities, knockdown of the hnRNP Q level elicited a dramatic decrease of peak amplitude in the AANAT protein profile parallel to reduced melatonin production in pinealocytes. This translational regulation of AANAT mRNA provides a novel aspect for achieving the circadian rhythmicity of vertebrate melatonin.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28494
- DOI
- 10.1101/GAD.1519507
- ISSN
- 0890-9369
- Article Type
- Article
- Citation
- GENES & DEVELOPMENT, vol. 21, no. 7, page. 797 - 810, 2007-04-01
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- There are no files associated with this item.
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