DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, MI | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Lee, J | - |
dc.contributor.author | Shin, S | - |
dc.contributor.author | Na, HB | - |
dc.contributor.author | Hyeon, T | - |
dc.contributor.author | Park, HG | - |
dc.contributor.author | Chang, HN | - |
dc.date.accessioned | 2016-04-01T08:51:35Z | - |
dc.date.available | 2016-04-01T08:51:35Z | - |
dc.date.created | 2009-06-01 | - |
dc.date.issued | 2008-04 | - |
dc.identifier.issn | 1387-1811 | - |
dc.identifier.other | 2008-OAK-0000016663 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/28925 | - |
dc.description.abstract | alpha-Chymotrypsin (CT) and lipase (LP) were immobilized in SBA-15 mesoporous silica by crosslinking adsorbed enzymes. This simple approach resulted in one-dimensional crosslinked enzyme aggregates (CLEAs) in the linear pore channels of SBA-15, which was very effective in preventing the enzyme leaching and consequently improving the enzyme stability. Both CLEAs of CT and LP showed negligible activity decrease under harsh shaking condition for one week while the conventional approaches including adsorption and covalent attachment resulted in more than 50-90% enzyme inactivation under the same condition. This effective stabilization results from the bent pore structure of SBA-15 with a high aspect ratio, which prevents the leaching of one-dimensional CLEAs and thereby achieves the higher enzyme loading capacity. Along with the higher specific activity than that of adsorbed enzymes, this CLEA approach is much simpler than that of covalent attachment by obviating the tedious processes for silica functionalization and enzyme attachment. (C) 2007 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | MICROPOROUS AND MESOPOROUS MATERIALS | - |
dc.title | One-dimensional crosslinked enzyme aggregates in SBA-15: Superior catalytic behavior to conventional enzyme immobilization | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/j.micromeso.2007.07.009 | - |
dc.author.google | Bin Na, H | - |
dc.author.google | Chang, HN | - |
dc.author.google | Hyeon, T | - |
dc.author.google | Kim, J | - |
dc.author.google | Kim, MI | - |
dc.author.google | Lee, J | - |
dc.author.google | Park, HG | - |
dc.author.google | Shin, S | - |
dc.relation.volume | 111 | - |
dc.relation.issue | 1-3 | - |
dc.relation.startpage | 18 | - |
dc.relation.lastpage | 23 | - |
dc.contributor.id | 10138815 | - |
dc.relation.journal | MICROPOROUS AND MESOPOROUS MATERIALS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MICROPOROUS AND MESOPOROUS MATERIALS, v.111, no.1-3, pp.18 - 23 | - |
dc.identifier.wosid | 000255847100005 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 23 | - |
dc.citation.number | 1-3 | - |
dc.citation.startPage | 18 | - |
dc.citation.title | MICROPOROUS AND MESOPOROUS MATERIALS | - |
dc.citation.volume | 111 | - |
dc.contributor.affiliatedAuthor | Lee, J | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 51 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ORDERED MESOPOROUS SILICA | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | BIOCATALYSIS | - |
dc.subject.keywordPlus | NANOFIBERS | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | LIPASE | - |
dc.subject.keywordAuthor | CLEAs (crosslinked enzyme aggregates) | - |
dc.subject.keywordAuthor | SBA-15 | - |
dc.subject.keywordAuthor | alpha-Chymotrypsin | - |
dc.subject.keywordAuthor | Mucor javanicus lipase | - |
dc.subject.keywordAuthor | enzyme immobilization | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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