Open Access System for Information Sharing

Login Library

 

Article
Cited 171 time in webofscience Cited 179 time in scopus
Metadata Downloads

Crosslinked enzyme aggregates in hierarchically-ordered mesoporous silica: A simple and effective method for enzyme stabilization SCIE SCOPUS

Title
Crosslinked enzyme aggregates in hierarchically-ordered mesoporous silica: A simple and effective method for enzyme stabilization
Authors
Kim, MIKim, JLee, JJia, HBin Na, HYoun, JKKwak, JHDohnalkova, AGrate, JWWang, PHyeon, TPark, HGChang, HN
Date Issued
2007-02-01
Publisher
JOHN WILEY & SONS INC
Abstract
alpha-chymotrypsin (CT) and lipase (LP) were immobilized in hierarchically-ordered mesocellular mesoporous silica (HMMS) in a simple but effective way for the enzyme stabilization, which was achieved by the enzyme adsorption followed by glutaraldehyde (GA) cross-linking. This resulted in the formation of nanometer scale crosslinked enzyme aggregates (CLEAs) entrapped in the mesocellular pores of HMMS (37 nm), which did not leach out of HMMS through narrow mesoporous channels (13 nm). CLEA of alpha-chymotrypsin (CLEA-CT) in HMMS showed a high enzyme loading capacity and significantly increased enzyme stability. No activity decrease of CLEA-CT was observed for 2 weeks under even rigorously shaking condition, while adsorbed CT in HMMS and free CT showed a rapid inactivation due to the enzyme leaching and presumably autolysis, respectively. With the CLEA-CT in HMMS, however, there was no tryptic digestion observed suggesting that the CLEA-CT is not susceptible to autolysis. Moreover, CLEA of lipase (CLEA-LP) in HMMS retained 30% specific activity of free lipase with greatly enhanced stability. This work demonstrates that HMMS can be efficiently employed as host materials for enzyme immobilization leading to highly enhanced stability of the immobilized enzymes with high enzyme loading and activity.
Keywords
CLEAs (crosslinked enzyme aggregates); alpha-chymotrypsin; Mucor javanicus lipase; enzyme immobilization; HMMS (hierarchically-ordered mesocellular mesoporous silica); CATALYTIC ACTIVITY; IMMOBILIZATION; PROTEINS; ACYLASE; ENCAPSULATION; BIOCATALYSIS; CHYMOTRYPSIN; CRYSTALS; SUPPORT; FOAMS
URI
https://oasis.postech.ac.kr/handle/2014.oak/28937
DOI
10.1002/BIT.21107
ISSN
0006-3592
Article Type
Article
Citation
BIOTECHNOLOGY AND BIOENGINEERING, vol. 96, no. 2, page. 210 - 218, 2007-02-01
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

이진우LEE, JIN WOO
Dept. of Chemical Enginrg
Read more

Views & Downloads

Browse