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Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata) SCIE SCOPUS
- Title
- Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata)
- Date Issued
- 1999-03-25
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLD alpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDa and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD. (C) 1999 Elsevier Science B.V. All rights reserved.
- Keywords
- phospholipase D; cDNA sequence; expression; (Brassica oleracea var. capitata); ADP-RIBOSYLATION FACTOR; D DEFINES; PLD-BETA; PURIFICATION; PHOSPHATIDYLCHOLINE; FAMILY; ALPHA; HYDROLYSIS; FORMS; GAMMA
- ISSN
- 1388-1981
- Article Type
- Article
- Citation
- BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, vol. 1437, no. 3, page. 409 - 414, 1999-03-25
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Related Researcher
- 노태영ROH, TAE YOUNG
- Dept of Life Sciences