Effect of organic solvents on penicillin acylase-catalyzed reactions: Interaction of organic solvents with enzymes
SCIE
SCOPUS
- Title
- Effect of organic solvents on penicillin acylase-catalyzed reactions: Interaction of organic solvents with enzymes
- Authors
- Kim, MG; Lee, SB
- Date Issued
- 1996-06-04
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- The effects of various organic solvents on penicillin acylase-catalyzed synthesis of p-lactam antibiotics (pivampicillin and ampicillin) have been investigated in water-solvent mixtures. The rates of penicillin acylase-catalyzed reactions were found to be significantly reduced by the presence of a small amount of organic solvent. In particular, the rate of enzyme catalysis was extremely low in the presence of ring-structured solvents and acids while enzyme activities were fully restored after removing the solvents. This indicates that interactions between the solvents and the enzyme are specific and reversible. To correlate the inhibitory effects of organic solvents with solvent properties the influence of solvent hydrophobicities and solvent activity on the rate of pivampicillin synthesis was examined. The reaction rate was found to decrease with increasing solvent hydrophobicities, and a better correlation was observed between the reaction rate and solvent activity. The effects of ionic strength on the synthesis of pivampicillin and ampicillin were also examined. The ionic strength dependence indicates that electrostatic interactions are involved in the binding of ionic compounds to the enzyme. On the basis of the active site structure of penicillin acylase, a possible mechanism for molecular interactions between the enzyme and organic solvents is suggested.
- Keywords
- penicillin acylase; pivampicillin; ampicillin; organic solvents; solvent hydrophobicity; thermodynamic activity; ionic strength; ESCHERICHIA-COLI; WATER; BIOCATALYSIS; COSOLVENTS; AMPICILLIN; AMIDASE; SET
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29274
- DOI
- 10.1016/1381-1177(96)00005-7
- ISSN
- 1381-1177
- Article Type
- Article
- Citation
- JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, vol. 1, no. 3-6, page. 181 - 190, 1996-06-04
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