Correlation analysis for non-invasive quantitative monitoring of biological activity of recombinant enzyme using green fluorescence protein in Escherichia coli under various culture environments
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- Title
- Correlation analysis for non-invasive quantitative monitoring of biological activity of recombinant enzyme using green fluorescence protein in Escherichia coli under various culture environments
- Authors
- Yoo, HJ; Seo, JH; Kang, DG; Cha, HJ
- Date Issued
- 2007-01
- Publisher
- KOREAN INST CHEM ENGINEERS
- Abstract
- Monitoring of biological activity for target enzyme is important for its production in recombinant expression systems. Previously, we demonstrated that green fluorescent protein (GFP) as a fusion partner is successfully tooled for facile, in vivo, and non-invasive quantification of target enzyme levels based on a linear relationship between GFP fluorescence and enzyme (chloramphenicol acetyltransferase; CAT) activity. Here, we investigated the effects of culture environmental variations (initial glucose amount, surface aeration, and inducer concentration) on correlation between GFP fluorescence and CAT activity, and established a general linear correlation as a unique criterion for quantitative monitoring of CAT biological activity. This general correlation for GFP fusion strategy can be applied for non-invasive and on-line monitoring of recombinant enzyme production under various culture conditions without further experimental calibrations.
- Keywords
- monitoring of biological activity; general linear correlation; green fluorescent protein; recombinant enzyme; fusion protein; Escherichia coli; GENE-EXPRESSION; FUSION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29456
- DOI
- 10.1007/s11814-007-5016-1
- ISSN
- 0256-1115
- Article Type
- Article
- Citation
- KOREAN JOURNAL OF CHEMICAL ENGINEERING, vol. 24, no. 1, page. 99 - 101, 2007-01
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