DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, YS | - |
dc.contributor.author | Seo, SW | - |
dc.contributor.author | Hwang, S | - |
dc.contributor.author | Chu, HS | - |
dc.contributor.author | Ahn, JH | - |
dc.contributor.author | Kim, TW | - |
dc.contributor.author | Kim, DM | - |
dc.contributor.author | Jung, GY | - |
dc.date.accessioned | 2016-04-01T09:07:33Z | - |
dc.date.available | 2016-04-01T09:07:33Z | - |
dc.date.created | 2009-03-13 | - |
dc.date.issued | 2007-04-27 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.other | 2007-OAK-0000010796 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29499 | - |
dc.description.abstract | Redesign or modification of the cellular physiology requires a quantitatively well-controlled expression system known as the "tunable expression." Although the modification of promoters demonstrates the great impact on the translation efficiency, it is difficult to detect the proper variants required for tunable expression. The 5'-untranslated region (UTR), however, can be an important target for tunable expressions because the ribosome binding affinity is directly modulated by the sequence variants of the Shine-Dalgarno (SD) sequence and the AU-rich sequence, which are the ribosome binding sites and a S13-sequence-independent translation enhancer, respectively. This study developed a simple method to obtain numerous 5'-UTR variants and analyze their translation efficiency based on the PCR-based site-directed mutagenesis and the expressional PCR using coupled in vitro transcription/translation system derived from Escherichia coli and eGFP gene as a template. SD sequence variants (18) and AU-rich sequence variants (36), which have a wide range of relative expression levels ranging from 0.1 to 2.0, were obtained. The translation efficiency was affected by the ribosome binding affinity and its accessibility that is dependent on the secondary structure around the 5'-UTR. (c) 2007 Elsevier Inc. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.subject | 5 &apos | - |
dc.subject | -UTR | - |
dc.subject | expressional PCR | - |
dc.subject | tunable expression | - |
dc.subject | SD sequence | - |
dc.subject | AU-rich sequence | - |
dc.subject | RIBOSOME-MESSENGER RECOGNITION | - |
dc.subject | RNA SECONDARY STRUCTURES | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | PROTEIN S1 | - |
dc.subject | TRANSLATION | - |
dc.subject | BINDING | - |
dc.subject | OPTIMIZATION | - |
dc.subject | SEQUENCES | - |
dc.subject | SITES | - |
dc.subject | TRANSCRIPTS | - |
dc.title | Design of 5 '-untranslated region variants for tunable expression in Escherichia coli | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.02.127 | - |
dc.author.google | Park, YS | - |
dc.author.google | Seo, SW | - |
dc.author.google | Hwang, S | - |
dc.author.google | Chu, HS | - |
dc.author.google | Ahn, JH | - |
dc.author.google | Kim, TW | - |
dc.author.google | Kim, DM | - |
dc.author.google | Jung, GY | - |
dc.relation.volume | 356 | - |
dc.relation.issue | 1 | - |
dc.relation.startpage | 136 | - |
dc.relation.lastpage | 141 | - |
dc.contributor.id | 10130678 | - |
dc.relation.journal | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.356, no.1, pp.136 - 141 | - |
dc.identifier.wosid | 000245419300023 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 141 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 136 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 356 | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-33947152402 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 21 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RNA SECONDARY STRUCTURES | - |
dc.subject.keywordPlus | PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | SEQUENCES | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | PROMOTERS | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | S1 | - |
dc.subject.keywordAuthor | 5 &apos | - |
dc.subject.keywordAuthor | -UTR | - |
dc.subject.keywordAuthor | expressional PCR | - |
dc.subject.keywordAuthor | tunable expression | - |
dc.subject.keywordAuthor | SD sequence | - |
dc.subject.keywordAuthor | AU-rich sequence | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.