DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dalal, NG | - |
dc.contributor.author | Cha, HJ | - |
dc.contributor.author | Kramer, SF | - |
dc.contributor.author | Kostov, Y | - |
dc.contributor.author | Rao, G | - |
dc.contributor.author | Bentley, WE | - |
dc.date.accessioned | 2016-04-01T09:09:11Z | - |
dc.date.available | 2016-04-01T09:09:11Z | - |
dc.date.created | 2009-08-13 | - |
dc.date.issued | 2006-04 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.other | 2006-OAK-0000010733 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29550 | - |
dc.description.abstract | We investigated monitoring for baculovirus infection of insect Trichoplusia ni larvae by combining the green fluorescent protein (GFP) reporter, the early-to-late (ETL) baculoviral promoter, and a GFP-specific optical probe. Because GFP is under the ETL promoter control, it facilitated rapid monitoring of recombinant baculovirus infection on insect larva system. Employment of GFP-specific optical probe also enabled non-invasive and accurate GFP monitoring with similar to 10 h prior to invasive and GFP Western blot assay. This combination may assist in monitoring protein production runs, determining optimal infection and harvest timing, and in general, help to increase the yield of recombinant proteins expressed in larvae. (c) 2005 Elsevier Ltd. All rights reserved. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.subject | baculovirus infection | - |
dc.subject | insect larvae | - |
dc.subject | early-to-late promoter | - |
dc.subject | green fluorescent protein | - |
dc.subject | GFP-specific optical probe | - |
dc.subject | non-invasive monitoring | - |
dc.subject | TRICHOPLUSIA-NI LARVAE | - |
dc.subject | HIGH-LEVEL EXPRESSION | - |
dc.subject | CELL-CULTURE | - |
dc.subject | VIRUS | - |
dc.subject | PURIFICATION | - |
dc.subject | VECTORS | - |
dc.subject | GENES | - |
dc.title | Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe | - |
dc.type | Article | - |
dc.contributor.college | 화학공학과 | - |
dc.identifier.doi | 10.1016/j.procbio.2005.10.020 | - |
dc.author.google | Dalal, NG | - |
dc.author.google | Cha, HJ | - |
dc.author.google | Kramer, SF | - |
dc.author.google | Kostov, Y | - |
dc.author.google | Rao, G | - |
dc.author.google | Bentley, WE | - |
dc.relation.volume | 41 | - |
dc.relation.issue | 4 | - |
dc.relation.startpage | 947 | - |
dc.relation.lastpage | 950 | - |
dc.contributor.id | 10057405 | - |
dc.relation.journal | PROCESS BIOCHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.41, no.4, pp.947 - 950 | - |
dc.identifier.wosid | 000236036800033 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 950 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 947 | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 41 | - |
dc.contributor.affiliatedAuthor | Cha, HJ | - |
dc.identifier.scopusid | 2-s2.0-33344476425 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 4 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TRICHOPLUSIA-NI LARVAE | - |
dc.subject.keywordPlus | HIGH-LEVEL EXPRESSION | - |
dc.subject.keywordPlus | CELL-CULTURE | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | VECTORS | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordAuthor | baculovirus infection | - |
dc.subject.keywordAuthor | insect larvae | - |
dc.subject.keywordAuthor | early-to-late promoter | - |
dc.subject.keywordAuthor | green fluorescent protein | - |
dc.subject.keywordAuthor | GFP-specific optical probe | - |
dc.subject.keywordAuthor | non-invasive monitoring | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
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