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Native sulfur/chlorine SAD phasing for serial femtosecond crystallography SCIE SCOPUS
- Title
- Native sulfur/chlorine SAD phasing for serial femtosecond crystallography
- Authors
- Nakane, T; Song, CY; Suzuki, M; Nango, E; Kobayashi, J; Masuda, T; Inoue, S; Mizohata, E; Nakatsu, T; Tanaka, T; Tanaka, R; Shimamura, T; Tono, K; Joti, Y; Kameshima, T; Hatsui, T; Yabashi, M; Nureki, O; Iwata, S; Sugahara, M
- Date Issued
- 2015-12
- Publisher
- IUCr
- Abstract
- Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 angstrom is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.
- ISSN
- 1399-0047
- Article Type
- Article
- Citation
- Acta Crystallographica Section D, vol. 71, no. 12, page. 2519 - 2525, 2015-12
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