Oil-free hyaluronic acid matrix for serial femtosecond crystallography
SCIE
SCOPUS
- Title
- Oil-free hyaluronic acid matrix for serial femtosecond crystallography
- Authors
- Sugahara, M; Song, C; Suzuki, M; Masuda, T; Inoue, S; Nakane, T; Yumoto, F; Nango, E; Tanaka, R; Tono, K; Joti, Y; Kameshima, T; Hatsui, T; Yabashi, M; Nureki, O; Numata, K; Iwata, S.
- Date Issued
- 2016-04-18
- Publisher
- NPG
- Abstract
- The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-angstrom resolution.
- Keywords
- FREE-ELECTRON LASER; X-RAY-DIFFRACTION; PROTEIN; SYSTEM; SACLA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29944
- DOI
- 10.1038/SREP24484
- ISSN
- 2045-2322
- Article Type
- Article
- Citation
- SCIENTIFIC REPORTS, vol. 6, page. 24484, 2016-04-18
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