DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, D | - |
dc.contributor.author | Hwang, DS | - |
dc.contributor.author | Kang Dong Gyun | - |
dc.contributor.author | Kim, JYH | - |
dc.contributor.author | Cha Hyung Joon | - |
dc.date.accessioned | 2017-07-18T16:41:37Z | - |
dc.date.available | 2017-07-18T16:41:37Z | - |
dc.date.created | 2010-11-04 | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/30957 | - |
dc.description.abstract | Mussel adhesive proteins (MAPs) have been considered as potential underwater and medical bioadhesives. Previously, we reported a functional expression of recombinant MAP hybrid fp-151, which is a fusion protein with six type 1 (fp-1) decapeptide repeats at each type 5 (fp-5) terminus, with practical properties in Escherichia coli. In the present work, we introduced the Vitreoscilla hemoglobin (VHb) co-expression strategy to enhance the production levels of hybrid fp-151 since VHb has been successfully used for efficient oxygen utilization in several expression systems, including E. coli. In both batch-type flask and fed-batch-type bioreactor cultures, we found that co-expression of VHb conferred higher cell growth and hybrid fp-151 production. Its positive effects were significantly increased in high cell density bioreactor cultures as the microaerobic environment was more quickly and severely formed. We obtained a similar to 1.9-fold higher (similar to 1 g/L) production of MAP fp-151 from VHb co-expressing cells in fed-batch bioreactor cultures as compared to that from VHb non-expressing cells. Collectively and regardless of the culture type, VHb co-expression strategy was successful in enhancing the production of recombinant mussel adhesive proteins in the E. coli expression system. | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | BIOTECHNOLOGY PROGRESS | - |
dc.subject | CHONDROCYTE TRANSPLANTATION SURGERY | - |
dc.subject | INDUCIBLE PROMOTER SYSTEM | - |
dc.subject | INACTIVATED NAR OPERON | - |
dc.subject | FED-BATCH CULTIVATION | - |
dc.subject | MYTILUS-EDULIS-L | - |
dc.subject | POLYPHENOLIC PROTEINS | - |
dc.subject | EXPRESSION | - |
dc.subject | VITREOSCILLA | - |
dc.subject | GENE | - |
dc.title | Enhancement of mussel adhesive protein production in Escherichia coli by co-expression of bacterial hemoglobin | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/bp0703477 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY PROGRESS, v.24, no.3, pp.663 - 666 | - |
dc.identifier.wosid | 000256593300024 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.endPage | 666 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 663 | - |
dc.citation.title | BIOTECHNOLOGY PROGRESS | - |
dc.citation.volume | 24 | - |
dc.contributor.affiliatedAuthor | Hwang, DS | - |
dc.contributor.affiliatedAuthor | Kang Dong Gyun | - |
dc.identifier.scopusid | 2-s2.0-45149101743 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 11 | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.subject.keywordPlus | CHONDROCYTE TRANSPLANTATION SURGERY | - |
dc.subject.keywordPlus | INDUCIBLE PROMOTER SYSTEM | - |
dc.subject.keywordPlus | INACTIVATED NAR OPERON | - |
dc.subject.keywordPlus | FED-BATCH CULTIVATION | - |
dc.subject.keywordPlus | MYTILUS-EDULIS-L | - |
dc.subject.keywordPlus | POLYPHENOLIC PROTEINS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | VITREOSCILLA | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
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