Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks
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SCOPUS
- Title
- Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks
- Authors
- Kim, C; Lee, OC; Kim, JY; Sung, W; Lee, NK
- Date Issued
- 2015-07-27
- Publisher
- John Wiley & Sons Ltd.
- Abstract
- Bending with high curvature is one of the major mechanical properties of double-stranded DNA (dsDNA) that is essential for its biological functions. The emergence of a kink arising from local melting in the middle of dsDNA has been suggested as a mechanism of releasing the energy cost of bending. Herein, we report that strong bending induces two types of short dsDNA deformations, induced by two types of local melting, namely, a kink in the middle and forks at the ends, which we demonstrate using D-shaped DNA nanostructures. The two types of deformed dsDNA structures dynamically interconvert on a millisecond timescale. The transition from a fork to a kink is dominated by entropic contribution (anti-Arrhenius behavior), while the transition from a kink to a fork is dominated by enthalpic contributions. The presence of mismatches in dsDNA accelerates kink formation, and the transition from a kink to a fork is removed when the mismatch size is three base pairs.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/35279
- DOI
- 10.1002/ANIE.201502055
- ISSN
- 1433-7851
- Article Type
- Article
- Citation
- Angewandte Chemie - International Edition, vol. 54, no. 31, page. 8943 - 8947, 2015-07-27
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