Enhancement of the catalytic activity of the wheat germ, cell-free, protein synthesis system using a fortified translation extract

Abstract

A wheat germ, cell-free, translation extract was fractionated into three concentrated parts using ammonium sulfate: the 0 - 40 % saturated fraction, the 40 - 60 % saturated fraction, and the ribosome fraction. These fractions were tested for their ability to enhance the translational activity of the wheat germ, cell-free extract for dihydrofolate reductase. The fortified cell-free system supplemented with the 0 - 40 % ammonium sulfate fraction enhanced the efficiency of protein synthesis by 50 %.