DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, J | - |
dc.contributor.author | Shin, H | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Park, J | - |
dc.date.accessioned | 2017-07-19T12:18:24Z | - |
dc.date.available | 2017-07-19T12:18:24Z | - |
dc.date.created | 2016-01-26 | - |
dc.date.issued | 2015-06-19 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/35603 | - |
dc.description.abstract | We present a simple and rapid method to isolate extracellular vesicles (EVs) by using a polyethylene glycol/dextran aqueous two-phase system (ATPS). This system isolated more than similar to 75% of melanoma-derived EVs from a mixture of EVs and serum proteins. To increase the purity of EVs, a batch procedure was combined as additional steps to remove protein contaminants, and removed more than similar to 95% of the protein contaminants. We also performed RT-PCR and western blotting to verify the diagnostic applicability of the isolated EVs, and detected mRNA derived from melanoma cells and CD81 in isolated EVs. | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLoS ONE | - |
dc.title | Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/JOURNAL.PONE.0129760 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PLoS ONE, v.10, no.6 | - |
dc.identifier.wosid | 000356835000056 | - |
dc.date.tcdate | 2019-03-01 | - |
dc.citation.number | 6 | - |
dc.citation.title | PLoS ONE | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Park, J | - |
dc.identifier.scopusid | 2-s2.0-84938918253 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 10 | - |
dc.description.scptc | 8 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MEMBRANE-VESICLES | - |
dc.subject.keywordPlus | PLASMA-MEMBRANES | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | INSIDE-OUT | - |
dc.subject.keywordPlus | EXOSOMES | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CELL | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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