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Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations SCIE SCOPUS

Title
Deformability measurement of red blood cells using a microfluidic channel array and an air cavity in a driving syringe with high throughput and precise detection of subpopulations
Authors
Kang, YJHa, YRLee, SJ
Date Issued
2016-01
Publisher
ROYAL SOC CHEMISTRY
Abstract
Red blood cell (RBC) deformability has been considered a potential biomarker for monitoring pathological disorders. High throughput and detection of subpopulations in RBCs are essential in the measurement of RBC deformability. In this paper, we propose a new method to measure RBC deformability by evaluating temporal variations in the average velocity of blood flow and image intensity of successively clogged RBCs in the microfluidic channel array for specific time durations. In addition, to effectively detect differences in subpopulations of RBCs, an air compliance effect is employed by adding an air cavity into a disposable syringe. The syringe was equally filled with a blood sample (V-blood = 0.3 mL, hematocrit = 50%) and air (V-air = 0.3 mL). Owing to the air compliance effect, blood flow in the microfluidic device behaved transiently depending on the fluidic resistance in the microfluidic device. Based on the transient behaviors of blood flows, the deformability of RBCs is quantified by evaluating three representative parameters, namely, minimum value of the average velocity of blood flow, clogging index, and delivered blood volume. The proposed method was applied to measure the deformability of blood samples consisting of homogeneous RBCs fixed with four different concentrations of glutaraldehyde solution (0%-0.23%). The proposed method was also employed to evaluate the deformability of blood samples partially mixed with normal RBCs and hardened RBCs. Thereafter, the deformability of RBCs infected by human malaria parasite Plasmodium falciparum was measured. As a result, the three parameters significantly varied, depending on the degree of deformability. In addition, the deformability measurement of blood samples was successfully completed in a short time (similar to 10 min). Therefore, the proposed method has significant potential in deformability measurement of blood samples containing hematological diseases with high throughput and precise detection of subpopulations in RBCs.
Keywords
MEASURING ERYTHROCYTE DEFORMABILITY; FALCIPARUM-INFECTED ERYTHROCYTES; SPONTANEOUSLY HYPERTENSIVE-RATS; PLASMODIUM-FALCIPARUM; SINGLE CELLS; VISCOSITY; FLOW; MEMBRANE; MICROCIRCULATION; SEDIMENTATION
URI
https://oasis.postech.ac.kr/handle/2014.oak/36150
DOI
10.1039/C5AN01988E
ISSN
0003-2654
Article Type
Article
Citation
ANALYST, vol. 141, no. 1, page. 319 - 330, 2016-01
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이상준LEE, SANG JOON
Dept of Mechanical Enginrg
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