DC Field | Value | Language |
---|---|---|
dc.contributor.author | Changhoon Song | - |
dc.contributor.author | Beom-Ju Hong | - |
dc.contributor.author | Seoyeon Bok | - |
dc.contributor.author | Chan-Ju Lee | - |
dc.contributor.author | Kim, YE | - |
dc.contributor.author | Sang-Rok Jeon | - |
dc.contributor.author | Hong-Gyun Wu | - |
dc.contributor.author | Yun-Sang Lee | - |
dc.contributor.author | Gi Jeong Cheon | - |
dc.contributor.author | Jin Chul Paeng | - |
dc.contributor.author | David J Carlson | - |
dc.contributor.author | Hak Jae Kim | - |
dc.contributor.author | Ahn, GO | - |
dc.date.accessioned | 2017-07-19T12:48:20Z | - |
dc.date.available | 2017-07-19T12:48:20Z | - |
dc.date.created | 2016-02-20 | - |
dc.date.issued | 2016-07-01 | - |
dc.identifier.issn | 0360-3016 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/36469 | - |
dc.description.abstract | Purpose: To investigate the serial changes of tumor hypoxia in response to single high-dose irradiation by various clinical and preclinical methods to propose an optimal fractionation schedule for stereotactic ablative radiation therapy. Methods and Materials: Syngeneic Lewis lung carcinomas were grown either orthotopically or subcutaneously in C57BL/6 mice and irradiated with a single dose of 15 Gy to mimic stereotactic ablative radiation therapy used in the clinic. Serial [F-18]-misonidazole (F-MISO) positron emission tomography (PET) imaging, pimonidazole fluorescence-activated cell sorting analyses, hypoxia-responsive element-driven bioluminescence, and Hoechst 33342 perfusion were performed before irradiation (day -1), at 6 hours (day 0), and 2 (day 2) and 6 (day 6) days after irradiation for both subcutaneous and orthotopic lung tumors. For F-MISO, the tumor/brain ratio was analyzed. Results: Hypoxic signals were too low to quantitate for orthotopic tumors using F-MISO PET or hypoxia-responsive element-driven bioluminescence imaging. In subcutaneous tumors, the maximum tumor/brain ratio was 2.87 +/- 0.483 at day -1, 1.67 +/- 0.116 at day 0, 2.92 +/- 0.334 at day 2, and 2.13 +/- 0.385 at day 6, indicating that tumor hypoxia was decreased immediately after irradiation and had returned to the pretreatment levels at day 2, followed by a slight decrease by day 6 after radiation. Pimonidazole analysis also revealed similar patterns. Using Hoechst 33342 vascular perfusion dye, CD31, and cleaved caspase 3 coimmunostaining, we found a rapid and transient vascular collapse, which might have resulted in poor intratumor perfusion of F-MISO PET tracer or pimonidazole delivered at day 0, leading to decreased hypoxic signals at day 0 by PET or pimonidazole analyses. Conclusions: We found tumor hypoxia levels decreased immediately after delivery of a single dose of 15 Gy and had returned to the pretreatment levels 2 days after irradiation and had decreased slightly by day 6. Our results indicate that single high-dose irradiation can produce a rapid, but reversible, vascular collapse in tumors. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | Elsevier | - |
dc.relation.isPartOf | International Jornal of Radiation Oncology, Biology, Physics | - |
dc.title | The real-time tumor oxygenation changes following a single high dose radiotherapy in orthotopic and subcutaneous lung cancers in mice: clinical implication for stereotactic ablative radiotherapy schedule optimization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ijrobp.2016.01.064 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | International Jornal of Radiation Oncology, Biology, Physics, v.95, no.3, pp.1022 - 1031 | - |
dc.identifier.wosid | 000377370600024 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 1031 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1022 | - |
dc.citation.title | International Jornal of Radiation Oncology, Biology, Physics | - |
dc.citation.volume | 95 | - |
dc.contributor.affiliatedAuthor | Ahn, GO | - |
dc.identifier.scopusid | 2-s2.0-84964589307 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 12 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | POSITRON-EMISSION-TOMOGRAPHY | - |
dc.subject.keywordPlus | PROSPECTIVE PHASE-II | - |
dc.subject.keywordPlus | STAGE-I | - |
dc.subject.keywordPlus | BODY RADIOTHERAPY | - |
dc.subject.keywordPlus | F-18 FLUOROMISONIDAZOLE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | REOXYGENATION | - |
dc.subject.keywordPlus | IRRADIATION | - |
dc.subject.keywordPlus | MODEL | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.