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Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs SCIE SCOPUS

Title
Effects of electromagnetic field frequencies on chondrocytes in 3D cell-printed composite constructs
Authors
Yi, HGKang, KSHong, JMJang, JPark, MNJeong, YHCho, DW
Date Issued
2016-07
Publisher
WILEY-BLACKWELL
Abstract
In cartilage tissue engineering, electromagnetic field (EMF) therapy has been reported to have a modest effect on promoting cartilage regeneration. However, these studies were conducted using different frequencies of EMF to stimulate chondrocytes. Thus, it is necessary to investigate the effect of EMF frequency on cartilage formation. In addition to the stimulation, a scaffold is required to satisfy the characteristics of cartilage such as its hydrated and dense extracellular matrix, and a mechanical resilience to applied loads. Therefore, we 3D-printed a composite construct composed of a polymeric framework and a chondrocyte-laden hydrogel. Here, we observed frequency-dependent positive and negative effects on chondrogenesis using a 3D cell-printed cartilage tissue. We found that a frequency of 45 Hz promoted gene expression and secretion of extracellular matrix molecules of chondrocytes. In contrast, a frequency of 7.5 Hz suppressed chondrogenic differentiation in vitro. Additionally, the EMF-treated composite constructs prior to implantation showed consistent results with those of in vitro, suggesting that in vitro pre-treatment with different EMF frequencies provides different capabilities for the enhancement of cartilage formation in vivo. This correlation between EMF frequency and 3D-printed chondrocytes suggests the necessity for optimization of EMF parameters when this physical stimulus is applied to engineered cartilage. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1797-1804, 2016.
URI
https://oasis.postech.ac.kr/handle/2014.oak/37005
DOI
10.1002/jbm.a.35714
ISSN
1549-3296
Article Type
Article
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, vol. 104, no. 7, page. 1797 - 1804, 2016-07
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조동우CHO, DONG WOO
Dept of Mechanical Enginrg
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