Development of a 3D cell printed construct considering angiogenesis for liver tissue engineering
SCIE
SCOPUS
- Title
- Development of a 3D cell printed construct considering angiogenesis for liver tissue engineering
- Authors
- Lee, JW; Choi, YJ; Yong, WJ; Pati, F; Shim, JH; Kang, KS; Kang, IH; PARK, JAE SUNG; Cho, DW
- Date Issued
- 2016-03
- Publisher
- IOP
- Abstract
- Several studies have focused on the regeneration of liver tissue in a two-dimensional (2D) planar environment, whereas actual liver tissue is three-dimensional (3D). Cell printing technology has been successfully utilized for building 3D structures; however, the poor mechanical properties of cell-laden hydrogels are a major concern. Here, we demonstrate the printing of a 3D cell-laden construct and its application to liver tissue engineering using 3D cell printing technology through a multi-head tissue/organ building system. Polycaprolactone (PCL) was used as a framework material because of its excellent mechanical properties. Collagen bioink containing three different types of cells-hepatocytes (HCs), human umbilical vein endothelial cells, and human lung fibroblasts-was infused into the canals of a PCL framework to induce the formation of capillary-like networks and liver cell growth. Aco-cultured 3D microenvironment of the three types of cells was successfully established and maintained. The vascular formation and functional abilities of HCs (i.e., albumin secretion and urea synthesis) demonstrated that the heterotypic interaction among HCs and nonparenchymal cells increased the survivability and functionality of HCs within the collagen gel. Therefore, our results demonstrate the prospect of using cell printing technology for the creation of heterotypic cellular interaction within a structure for liver tissue engineering.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37469
- DOI
- 10.1088/1758-5090/8/1/015007
- ISSN
- 1758-5082
- Article Type
- Article
- Citation
- Biofabrication, vol. 8, no. 1, 2016-03
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.