Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying
SCIE
SCOPUS
- Title
- Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying
- Authors
- Prywes, N; Michaels, YS; Pal, A; Oh, SS; Szostak, JW
- Date Issued
- 2016-04
- Publisher
- Royal Society of Chemistry
- Abstract
- Ribozyme-catalyzed RNA polymerization is inefficient and error prone. Here we demonstrate that two alternative bases, 2-thio-uridine (s(2)U) and 2-thio-ribo-thymidine (s(2)T), improve the rate and fidelity of ribozyme catalyzed nucleotide addition as NTP substrates and as template bases. We also demonstrate the functionality of s(2)U and s(2)T-containing ribozymes.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/37706
- DOI
- 10.1039/C6CC02692C
- ISSN
- 1359-7345
- Article Type
- Article
- Citation
- CHEMICAL COMMUNICATIONS, vol. 52, no. 39, page. 6529 - 6532, 2016-04
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