DC Field | Value | Language |
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dc.contributor.author | Oh, SS | - |
dc.contributor.author | Plakos, K | - |
dc.contributor.author | Lou, XH | - |
dc.contributor.author | Xiao, Y | - |
dc.contributor.author | Soh, HT | - |
dc.date.accessioned | 2017-07-19T13:50:58Z | - |
dc.date.available | 2017-07-19T13:50:58Z | - |
dc.date.created | 2017-02-27 | - |
dc.date.issued | 2010-08-10 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/37728 | - |
dc.description.abstract | We describe an innovative selection approach to generate self-reporting aptamers (SRAs) capable of converting target-binding events into fluorescence readout without requiring additional modification, optimization, or the use of DNA helper strands. These aptamers contain a DNAzyme moiety that is initially maintained in an inactive conformation. Upon binding to their target, the aptamers undergo a structural switch that activates the DNAzyme, such that the binding event can be reported through significantly enhanced fluorescence produced by a specific stacking interaction between the active-conformation DNAzyme and a small molecule dye, N-methylmesoporphyrin IX. We demonstrate a purely in vitro selection-based approach for obtaining SRAs that function in both buffer and complex mixtures such as blood serum; after 15 rounds of selection with a structured DNA library, we were able to isolate SRAs that possess low nanomolar affinity and strong specificity for thrombin. Given ongoing progress in the engineering and characterization of functional DNA/RNA molecules, strategies such as ours have the potential to enable rapid, efficient, and economical isolation of nucleic acid molecules with diverse functionalities. | - |
dc.language | English | - |
dc.publisher | National Academy of Sciences | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.title | In vitro selection of structure-switching, self-reporting aptamers | - |
dc.type | Article | - |
dc.identifier.doi | 10.1073/PNAS.1009172107 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.107, no.32, pp.14053 - 14058 | - |
dc.identifier.wosid | 000280767700017 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 14058 | - |
dc.citation.number | 32 | - |
dc.citation.startPage | 14053 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 107 | - |
dc.contributor.affiliatedAuthor | Oh, SS | - |
dc.identifier.scopusid | 2-s2.0-77956290872 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 63 | - |
dc.description.scptc | 61 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | SIGNALING APTAMERS | - |
dc.subject.keywordPlus | MICROMAGNETIC SELECTION | - |
dc.subject.keywordPlus | MOLECULAR RECOGNITION | - |
dc.subject.keywordPlus | EQUILIBRIUM MIXTURES | - |
dc.subject.keywordPlus | BLOOD-SERUM | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordAuthor | G-quadruplex | - |
dc.subject.keywordAuthor | systematic evolution of ligands by exponential enrichment | - |
dc.subject.keywordAuthor | sensor | - |
dc.subject.keywordAuthor | binding induced folding | - |
dc.subject.keywordAuthor | molecular recognition | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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