In-vivo analysis of uptake process of heavy metals through maize roots using synchrotron X-ray fluorescence spectroscopy
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- Title
- In-vivo analysis of uptake process of heavy metals through maize roots using synchrotron X-ray fluorescence spectroscopy
- Authors
- Bae Geun Hwang; Sang Joon Lee; Kyehwan Gil
- Date Issued
- 2016-12
- Publisher
- KOREAN PHYSICAL SOC
- Abstract
- The uptake of heavy metals by plants has been receiving much attention for crop contamination and phytoremediation. We employed synchrotron X-ray fluorescence (XRF) spectroscopy for an in-vivo analysis of heavy-metal uptake through a strand of maize root. A focused X-ray beam of 2.5 x 2.5 mu m(2) in physical dimensions was scanned along horizontal lines of the maize root at intervals of 3 mu m at the 4B X-ray micro-diffraction beamline of the Pohang Accelerator Laboratory (PAL). Time-resolved mapping of the fluorescence intensities from multiple metallic elements in the root tissues provided information about the radial distributions of heavy-metal elements and their temporal variations. The concentrated core stream of heavy-metal elements spread radially up to roughly 500 mu m, corresponding to 40 % of the root diameter. The absorption characteristics of three heavy metals, Cr, Mn and Ni, and their physiological features were analyzed. The absolute concentrations and the contents of the heavy-metal elements in the tested maize roots were quantitatively evaluated by using the calibration curve obtained from reference samples with preset concentrations. The uptake quantities of the tested heavy-metal elements are noticeably different, although their molecular weights are similar. This study should be helpful for understanding plant physiology related with heavy-metal uptake.
- Keywords
- XRF spectroscopy; Heavy metal; Phytoremediation
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/38232
- DOI
- 10.3938/jkps.69.1824
- ISSN
- 0374-4884
- Article Type
- Article
- Citation
- JOURNAL OF THE KOREAN PHYSICAL SOCIETY, vol. 69, no. 12, page. 1824 - 1829, 2016-12
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