A three-dimensional movie of structural changes in bacteriorhodopsin
SCIE
SCOPUS
- Title
- A three-dimensional movie of structural changes in bacteriorhodopsin
- Authors
- Nango, E; Royant, A; Kubo, M; Nakane, T; Wickstrand, C; Kimura, T; Tanaka, T; Tono, K; Song, CY; Tanaka, R; Arima, T; Yamashita, A; Kobayashi, J; Hosaka, T; Mizohata, E; Nogly, P; Sugahara, M; Nam, D; Nomura, T; Shimamura, T; Im, D; Fujiwara, T; Yamanaka, Y; Jeon, B; Nishizawa, T; Oda, K; Fukuda, M; Andersson, R; Bath, P; Dods, R; Davidsson, J; Matsuoka, S; Kawatake, S; Murata, M; Nureki, O; Owada, S; Kameshima, T; Hatsui, T; Joti, Y; Schertler, G; Yabashi, M; Bondar, AN; Standfuss, J; Neutze, R; Iwata, S
- Date Issued
- 2016-12-23
- Publisher
- AMER ASSOC ADVANCEMENT SCIENCE
- Abstract
- Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/39228
- DOI
- 10.1126/science.aah3497
- ISSN
- 0036-8075
- Article Type
- Article
- Citation
- SCIENCE, vol. 354, no. 6319, page. 1552 - 1557, 2016-12-23
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- There are no files associated with this item.
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