DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, JW | - |
dc.contributor.author | Woo, JM | - |
dc.contributor.author | Jung, GY | - |
dc.contributor.author | Bornscheuer, UT | - |
dc.contributor.author | Park, JB | - |
dc.date.accessioned | 2018-01-04T11:20:49Z | - |
dc.date.available | 2018-01-04T11:20:49Z | - |
dc.date.created | 2017-07-05 | - |
dc.date.issued | 2016-07-11 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/39254 | - |
dc.description.abstract | 3'-Untranslated region (3'UTR) engineering was investigated to improve solubility of heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of gene fragments containing putative RNase E recognition sites into the 3'UTR of the BVMO genes led to the reduction of mRNA levels in E. coli. Importantly, the amounts of soluble BVMOs were remarkably enhanced resulting in a proportional increase of in vivo catalytic activities. Notably, this increase in biocatalytic activity correlated to the number of putative RNase E endonucleolytic cleavage sites in the 3'UTR. For instance, the biotransformation activity of the BVMO BmoF1 (from Pseudomonas fluorescens DSM50106) in E. coli was linear to the number of RNase E cleavage sites in the 3'UTR. In summary, 3'UTR engineering can be used to improve the soluble expression of heterologous enzymes, thereby fine-tuning the enzyme activity in microbial cells. | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | Scientific Reports | - |
dc.title | 3′-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/SREP29406 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Scientific Reports, v.6 | - |
dc.identifier.wosid | 000379388400001 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.title | Scientific Reports | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Jung, GY | - |
dc.identifier.scopusid | 2-s2.0-84978884579 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 8 | - |
dc.description.scptc | 4 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BAEYER-VILLIGER MONOOXYGENASE | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN EXPRESSION | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | MICROBIAL SYNTHESIS | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | UNTRANSLATED REGION | - |
dc.subject.keywordPlus | DIRECTED EVOLUTION | - |
dc.subject.keywordPlus | SYNTHETIC BIOLOGY | - |
dc.subject.keywordPlus | BIOTRANSFORMATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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