CD82/KAI1 Maintains the Dormancy of Long-Term Hematopoietic Stem Cells through Interaction with DARC- Expressing Macrophages
SCIE
SCOPUS
- Title
- CD82/KAI1 Maintains the Dormancy of Long-Term Hematopoietic Stem Cells through Interaction with DARC- Expressing Macrophages
- Authors
- Jin Hur; Jae-Il Choi; Hwan Lee; Pniel Nham; Tae-Won Kim; Cheong-Whan Chae; Ji-Yeon Yun; Jin-A Kang; Jeehoon Kang; Sang Eun Lee; Chang-Hwan Yoon; Kyungjin Boo; Seokjin Ham; ROH, TAE YOUNG; Jong Kwan Jun; Ho Lee; Sung Hee Baek; Hyo-Soo Kim
- Date Issued
- 2016-04-07
- Publisher
- Cell Press
- Abstract
- Hematopoiesis is regulated by crosstalk between long-term repopulating hematopoietic stem cells (LT-HSCs) and supporting niche cells in the bone marrow (BM). Here, we examine the role of CD82/ KAI1 in niche-mediated LT-HSC maintenance. We found that CD82/ KAI1 is expressed predominantly on LT-HSCs and rarely on other hematopoietic stem-progenitor cells (HSPCs). In Cd82 +/-/+/- mice, LTHSCs were selectively lost as they exited from quiescence and differentiated. Mechanistically, CD82based TGF-b1/ Smad3 signaling leads to induction of CDK inhibitors and cell-cycle inhibition. The CD82 binding partner DARC/ CD234 is expressed on macrophages and stabilizes CD82 on LT-HSCs, promoting their quiescence. When DARC + BMmacrophages were ablated, the level of surface CD82 on LT-HSCs decreased, leading to cell-cycle entry, proliferation, and differentiation. A similar interaction appears to be relevant for human HSPCs. Thus, CD82 is a functional surface marker of LT-HSCs that maintains quiescence through interaction with DARC-expressing macrophages in the BM stem cell niche.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/39273
- DOI
- 10.1016/j.stem.2016.01.013
- ISSN
- 1934-5909
- Article Type
- Article
- Citation
- Cell Stem Cell, vol. 18, no. 7, page. 508 - 21, 2016-04-07
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