DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lapkouski, M | - |
dc.contributor.author | Chuenchor, W | - |
dc.contributor.author | Kim, MS | - |
dc.contributor.author | Gellert, M | - |
dc.contributor.author | Yang, W | - |
dc.date.accessioned | 2018-01-04T13:22:02Z | - |
dc.date.available | 2018-01-04T13:22:02Z | - |
dc.date.created | 2017-08-03 | - |
dc.date.issued | 2015-06-05 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/39357 | - |
dc.description.abstract | Mammalian immune receptor diversity is established via a unique restricted set of site-specific DNA rearrangements in lymphoid cells, known as V(D)J recombination. The lymphoid-specific RAG1-RAG2 protein complex (RAG1/2) initiates this process by binding to two types of recombination signal sequences (RSS), 12RSS and 23RSS, and cleaving at the boundaries of RSS and V, D, or J gene segments, which are to be assembled into immunoglobulins and T-cell receptors. Here we dissect the ordered assembly of the RAG1/2 heterotetramer with 12RSS and 23RSS DNAs. We find that RAG1/2 binds only a single 12RSS or 23RSS and reserves the second DNA-binding site specifically for the complementary RSS, to form a paired complex that reflects the known 12/23 rule of V(D)J recombination. The assembled RAG1/2 paired complex is active in the presence of Mg2+, the physiologically relevant metal ion, in nicking and double-strand cleavage of both RSS DNAs to produce a signal-end complex. We report here the purification and initial crystallization of the RAG1/2 signal-end complex for atomic-resolution structure elucidation. Strict pairing of the 12RSS and 23RSS at the binding step, together with information from the crystal structure of RAG1/2, leads to a molecular explanation of the 12/23 rule. | - |
dc.language | English | - |
dc.publisher | American Society for biochemistry and molecular biology | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.title | Assembly Pathway and Characterization of the RAG1/2-DNA Paired and Signal-end Complexes | - |
dc.type | Article | - |
dc.identifier.doi | 10.1074/jbc.M115.641787 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.290, no.23, pp.14618 - 14625 | - |
dc.identifier.wosid | 000355754600032 | - |
dc.citation.endPage | 14625 | - |
dc.citation.number | 23 | - |
dc.citation.startPage | 14618 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 290 | - |
dc.contributor.affiliatedAuthor | Kim, MS | - |
dc.identifier.scopusid | 2-s2.0-84930631577 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | V(D)J RECOMBINATION | - |
dc.subject.keywordPlus | SYNAPTIC COMPLEX | - |
dc.subject.keywordPlus | SOMATIC RECOMBINATION | - |
dc.subject.keywordPlus | ACTIVE-SITE | - |
dc.subject.keywordPlus | CHAIN GENES | - |
dc.subject.keywordPlus | RAG1 | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SYNAPSIS | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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