DC Field | Value | Language |
---|---|---|
dc.contributor.author | YUN, EUN JIN | - |
dc.contributor.author | Yoon, Wan-Hee | - |
dc.contributor.author | Lee, Sang-Kwang | - |
dc.contributor.author | Song, Kyoungsub | - |
dc.contributor.author | Kim, Jong-Seok | - |
dc.contributor.author | Kim, Tae-Dong | - |
dc.contributor.author | Li, Ge | - |
dc.contributor.author | Heo, Jun-Young | - |
dc.contributor.author | Jung, Yeon-Joo | - |
dc.contributor.author | Park, Jong-Il | - |
dc.contributor.author | Kweon, Gi Ryang | - |
dc.contributor.author | Koo, Sun-Hoe | - |
dc.contributor.author | Park, Hae-Duck | - |
dc.contributor.author | Hwang, Byung-Doo | - |
dc.contributor.author | Lim, Kyu | - |
dc.date.accessioned | 2018-01-09T09:04:03Z | - |
dc.date.available | 2018-01-09T09:04:03Z | - |
dc.date.created | 2017-11-14 | - |
dc.date.issued | 2008-09 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/40846 | - |
dc.description.abstract | It has been reported that the SW480 human colon cancer cell line consists of E-type and R-type cells. The long-term tumorigenic potential, invasive and metastatic properties of these subclones have not been characterized. E-type and R-type cells were subcloned using limiting dilution methods from parental SW480 cells. The cell growth rate was determined by MTT colorimetric assay, and colony forming efficiency was analyzed using Matrigel-coated plates. The activity of matrix metalloproteinase (MMP) and of urokinase plasminogen activator (uPA) was assessed by zymography. Invasive and locomotive ability was analyzed using trans-well chambers. In situ apoptosis detection of these subclones was also performed. In vivo long-term tumorigenicity and nodal metastasis were evaluated using nude mice. E-type cells produced spontaneously regressive tumors in spite of invasion and lymph node metastasis. In contrast, R-type cells revealed progressively growing tumors without invasion or metastasis. E-type cells exhibited increased apoptosis and invasive and motile ability, as well as strong MMP-9 and -2 activity. Although phorbol 12-myristate 13-acetate treatment induced MMP-9 activity in E-type cells, it had no effect on R-type cells. These findings suggest that E-type and R-type cells may have different biological properties in terms of colon cancer progression, regression, invasion and nodal metastasis, and might serve as a useful model for these studies. | - |
dc.language | English | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.relation.isPartOf | Molecular Medicine Reports | - |
dc.subject | GENETIC INSTABILITY | - |
dc.subject | FACTOR-BETA | - |
dc.subject | GROWTH | - |
dc.subject | MUTATIONS | - |
dc.subject | CARCINOMAS | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEIN | - |
dc.subject | TUMORS | - |
dc.subject | LUNG | - |
dc.title | The tumorigenic, invasive and metastatic potential of epithelial and round subpopulations of the SW480 human colon cancer cell line | - |
dc.type | Article | - |
dc.identifier.doi | 10.3892/mmr_00000026 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Molecular Medicine Reports, v.1, no.5, pp.763 - 768 | - |
dc.identifier.wosid | 000261058200026 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 768 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 763 | - |
dc.citation.title | Molecular Medicine Reports | - |
dc.citation.volume | 1 | - |
dc.contributor.affiliatedAuthor | YUN, EUN JIN | - |
dc.identifier.scopusid | 2-s2.0-69449096800 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 7 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | GENETIC INSTABILITY | - |
dc.subject.keywordPlus | FACTOR-BETA | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | LUNG | - |
dc.subject.keywordAuthor | SW480 | - |
dc.subject.keywordAuthor | E-type and R-type cells | - |
dc.subject.keywordAuthor | spontaneous regression | - |
dc.subject.keywordAuthor | invasion | - |
dc.subject.keywordAuthor | metastasis | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
library@postech.ac.kr Tel: 054-279-2548
Copyrights © by 2017 Pohang University of Science ad Technology All right reserved.