DC Field | Value | Language |
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dc.contributor.author | YUN, EUN JIN | - |
dc.contributor.author | Kim, Jong-Seok | - |
dc.contributor.author | Lee, Young-Chul | - |
dc.contributor.author | Nam, Ho-Tak | - |
dc.contributor.author | Li, Ge | - |
dc.contributor.author | Song, Kyoungsub | - |
dc.contributor.author | Seo, Kang-Sik | - |
dc.contributor.author | Park, Ji-Hoon | - |
dc.contributor.author | Ahn, Jong-Woong | - |
dc.contributor.author | Zee, Okpyo | - |
dc.contributor.author | Park, Jong-Il | - |
dc.contributor.author | Yoon, Wan-Hee | - |
dc.contributor.author | Lim, Kyu | - |
dc.contributor.author | Hwang, Byung-Doo | - |
dc.date.accessioned | 2018-01-09T09:04:06Z | - |
dc.date.available | 2018-01-09T09:04:06Z | - |
dc.date.created | 2017-11-14 | - |
dc.date.issued | 2007-11-01 | - |
dc.identifier.issn | 1078-0432 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/40848 | - |
dc.description.abstract | Purpose: Apicularen A has been shown to cause growth inhibition and apoptosis in several cancer cell lines. However, the mechanisms of apicularen A-induced cell death and in vivo effects remain unclear. In this study, we investigated the molecular mechanisms of apicularen A-induced cell death in HM7 human colon cancer cells in vitro and anticancer activity in vivo. Experimental Design: We tested cytotoxicity with a 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide, apoptosis with DNA fragmentation assay, mitochondrial membrane potential, and cell cycle with fluorescence-activated cell sorting. Caspase activation was done by fluorometry. Alterations of microtubule structure, tubulin protein, and mRNA level were assessed by immunofluorescence, Western blot, and reverse transcription- PCR. In vivo studies were assessed using nude mice tumor cell growth in xenograft model and liver colonization assay. Results: Apicularen A treatment of HM7 cells inhibited cell growth and this inhibition was partially rescued by z-VAD-fmk. Apicularen A caused accumulation of sub-G(1)-G(0), DNA fragmentation, Fas ligand induction, and activation of caspase-8 and caspase-3, but mitochondrial membrane potential was not changed. Furthermore, beta-tubulin protein and mRNAwere decreased by apicularen A, but in vitro polymerization of tubulin was not affected. Concurrently, apicularen A -treated cell showed disruption of microtubule architecture. In in vivo studies, apicularen A reduced tumor volume by similar to 72% at the end of a 15-day treatment. Moreover, apicularen A reduced liver colonization as much as 95.6% (50 mu g/kg/d). Conclusion: Apicularen A induces cell death of HM7 cells through up-regulating Fas ligand and disruption of microtubule architecture with down-regulation of tubulin level. These findings indicate that apicularen A is a promising new microtubule-targeting compound. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC CANCER RESEARCH | - |
dc.relation.isPartOf | CLINICAL CANCER RESEARCH | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | RAW-264.7 CELLS | - |
dc.subject | CARCINOMA-CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | ACTIVATION | - |
dc.subject | INDUCTION | - |
dc.subject | GROWTH | - |
dc.subject | LINE | - |
dc.subject | METASTASIS | - |
dc.subject | CASPASE | - |
dc.title | Apicularen A induces cell death through Fas ligand up-regulation and microtubule disruption by tubulin down-regulation in HM7 human colon cancer cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1158/1078-0432.CCR-07-1428 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | CLINICAL CANCER RESEARCH, v.13, no.21, pp.6509 - 6517 | - |
dc.identifier.wosid | 000250777600032 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 6517 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 6509 | - |
dc.citation.title | CLINICAL CANCER RESEARCH | - |
dc.citation.volume | 13 | - |
dc.contributor.affiliatedAuthor | YUN, EUN JIN | - |
dc.identifier.scopusid | 2-s2.0-35948931409 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 18 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | RAW-264.7 CELLS | - |
dc.subject.keywordPlus | CARCINOMA-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | CASPASE | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
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