DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tian, Lan | - |
dc.contributor.author | KIM, MIN SUNG | - |
dc.contributor.author | Li, Hongzhi | - |
dc.contributor.author | Wang, Jimin | - |
dc.contributor.author | Yang, Wei | - |
dc.date.accessioned | 2018-05-03T09:36:10Z | - |
dc.date.available | 2018-05-03T09:36:10Z | - |
dc.date.created | 2018-02-28 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/41007 | - |
dc.description.abstract | HIV-1 reverse transcriptase (RT) contains both DNA polymerase and RNase H activities to convert the viral genomic RNA to dsDNA in infected host cells. Here we report the 2.65-angstrom resolution structure of HIV-1 RT engaging in cleaving RNA in an RNA/DNA hybrid. A preferred substrate sequence is absolutely required to enable the RNA/DNA hybrid to adopt the distorted conformation needed to interact properly with the RNase H active site in RT. Substituting two nucleotides 4 bp upstream from the cleavage site results in scissile-phosphate displacement by 4 angstrom. We also have determined the structure of HIV-1 RT complexed with an RNase H-resistant polypurine tract sequence, which adopts a rigid structure and is accommodated outside of the nuclease active site. Based on this newly gained structural information and a virtual drug screen, we have identified an inhibitor specific for the viral RNase H but not for its cellular homologs. | - |
dc.language | English | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES | - |
dc.title | Structure of HIV-1 reverse transcriptase cleaving RNA in an RNA/DNA hybrid | - |
dc.type | Article | - |
dc.identifier.doi | 10.1073/pnas.1719746115 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES, v.115, no.3, pp.507 - 512 | - |
dc.identifier.wosid | 000423091400043 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 512 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 507 | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-PHYSICAL SCIENCES | - |
dc.citation.volume | 115 | - |
dc.contributor.affiliatedAuthor | KIM, MIN SUNG | - |
dc.identifier.scopusid | 2-s2.0-85042093478 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RIBONUCLEASE-H | - |
dc.subject.keywordPlus | SUBSTRATE-SPECIFICITY | - |
dc.subject.keywordPlus | ANGSTROM RESOLUTION | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURES | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | REQUIREMENTS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordAuthor | sequence specificity | - |
dc.subject.keywordAuthor | polypurine tract | - |
dc.subject.keywordAuthor | minor groove recognition | - |
dc.subject.keywordAuthor | connection domain | - |
dc.subject.keywordAuthor | RNase H | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
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