The vital dye CDr10b labels the Zebrafish mid-intestine and lumen
SCIE
SCOPUS
- Title
- The vital dye CDr10b labels the Zebrafish mid-intestine and lumen
- Authors
- Sander, Veronika; Patke, Shantanu; Lee, Jung Y; CHANG, YOUNG TAE; Davidson, Alan J.
- Date Issued
- 2017-03
- Publisher
- MDPI
- Abstract
- We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals.
- Keywords
- zebrafish; gut development; mid-intestine; CDr10b; vital dye; laser ablation
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/41093
- DOI
- 10.3390/molecules22030454
- ISSN
- 1420-3049
- Article Type
- Article
- Citation
- MOLECULES, vol. 22, no. 3, page. 454, 2017-03
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- There are no files associated with this item.
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