Identification of a novel protein regulating microtubule stability through a chemical approach
SCIE
SCOPUS
- Title
- Identification of a novel protein regulating microtubule stability through a chemical approach
- Authors
- Wignall, SM; Gray, NS; Chang, Young-Tae; Juarez, L; Jacob, R; Burlingame, A; Schultz, PG; Heald, R
- Date Issued
- 2004-01
- Publisher
- CELL PRESS
- Abstract
- To identify novel proteins regulating the microtubule cytoskeleton, we screened a library of purine derivatives using mitotic spindle assembly in Xenopus egg extracts as an assay. Out of a collection of 1561 compounds, we identified 15 that destabilized microtubules without targeting tubulin directly, resulting in small spindles. Affinity chromatography with one compound named diminutol, revealed a potential target as NQO1: an NADP-dependent oxidoreductase. A role for NQO1 in influencing microtubule polymerization was confirmed through inhibition studies using known inhibitors and immunodepletion. Therefore, this chemical approach has identified a novel factor required for microtubule morphogenesis and cell division.
- Keywords
- CULTURED-MAMMALIAN-CELLS; SMALL-MOLECULE INHIBITOR; INDUCED OXIDATIVE STRESS; XENOPUS EGG EXTRACTS; INTRACELLULAR TARGETS; MITOTIC APPARATUS; KINASE INHIBITORS; DT-DIAPHORASE; IN-VITRO; BINDING
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50175
- DOI
- 10.1016/j.chembiol.2003.12.019
- ISSN
- 1074-5521
- Article Type
- Article
- Citation
- CHEMISTRY & BIOLOGY, vol. 11, no. 1, page. 135 - 146, 2004-01
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- There are no files associated with this item.
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