A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons
SCIE
SCOPUS
- Title
- A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons
- Authors
- Kim, Beomsue; Feng, Suihan; Yun, Seong-Wook; Leong, Cheryl; Satapathy, Rudrakanta; Wan, Si Yan Diana; Chang, Young-Tae
- Date Issued
- 2016-11
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2(low) cells from heterogeneous cell populations.
- Keywords
- STEM-CELLS; CHEMICAL PROBE; BINDING
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50361
- DOI
- 10.1002/cbic.201600490
- ISSN
- 1439-4227
- Article Type
- Article
- Citation
- CHEMBIOCHEM, vol. 17, no. 22, page. 2118 - 2122, 2016-11
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- There are no files associated with this item.
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