DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sim, Ji Hyun | - |
dc.contributor.author | Kim, Kyung Soo | - |
dc.contributor.author | Park, Hyoungjun | - |
dc.contributor.author | Kim, Kyung-Jin | - |
dc.contributor.author | Lin, Haiyue | - |
dc.contributor.author | Kim, Tae-Joo | - |
dc.contributor.author | Shin, Hyun Mu | - |
dc.contributor.author | Kim, Gwanghun | - |
dc.contributor.author | Lee, Dong-Sup | - |
dc.contributor.author | Park, Chan-Wook | - |
dc.contributor.author | Lee, Dong Hun | - |
dc.contributor.author | Kang, Insoo | - |
dc.contributor.author | Kim, Sung Joon | - |
dc.contributor.author | Cho, Chung-Hyun | - |
dc.contributor.author | Doh, Junsang | - |
dc.contributor.author | Kim, Hang-Rae | - |
dc.date.accessioned | 2018-06-15T05:21:52Z | - |
dc.date.available | 2018-06-15T05:21:52Z | - |
dc.date.created | 2017-09-14 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 1664-3224 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/50400 | - |
dc.description.abstract | The voltage-gated potassium channel, Kv1.3, and the Ca2+-activated potassium channel, KCa3.1, regulate membrane potentials in T cells, thereby controlling T cell activation and cytokine production. However, little is known about the expression and function of potassium channels in human effector memory ( EM) CD8+ T cells that can be further divided into functionally distinct subsets based on the expression of the interleukin ( IL)-7 receptor alpha ( IL-7R alpha) chain. Herein, we investigated the functional expression and roles of Kv1.3 and KCa3.1 in EM CD8+ T cells that express high or low levels of the IL-7 receptor alpha chain ( IL-7R alpha(high) and IL-7R alpha(low), respectively). In contrast to the significant activity of Kv1.3 and KCa3.1 in IL-7Rahigh EM CD8+ T cells, IL-7Ralow EM CD8+ T cells showed lower expression of Kv1.3 and insignificant expression of KCa3.1. Kv1.3 was involved in the modulation of cell proliferation and IL-2 production, whereas KCa3.1 affected the motility of EM CD8+ T cells. The lower motility of IL-7Ralow EM CD8+ T cells was demonstrated using transendothelial migration and motility assays with intercellular adhesion molecule 1-and/or chemokine stromal cell-derived factor-1 alpha-coated surfaces. Consistent with the lower migration property, IL-7Ralow EM CD8+ T cells were found less frequently in human skin. Stimulating IL-7Ralow EM CD8+ T cells with IL-2 or IL-15 increased their motility and recovery of KCa3.1 activity. Our findings demonstrate that Kv1.3 and KCa3.1 are differentially involved in the functions of EM CD8+ T cells. The weak expression of potassium channels in IL-7Ralow EM CD8+ T cells can be revived by stimulation with IL-2 or IL-15, which restores the associated functions. This study suggests that IL-7Rahigh EM CD8+ T cells with functional potassium channels may serve as a reservoir for effector CD8+ T cells during peripheral inflammation. | - |
dc.language | English | - |
dc.publisher | Frontiers Media S.A. | - |
dc.relation.isPartOf | Frontiers in Immunology | - |
dc.title | Differentially Expressed Potassium Channels Are Associated with Function of Human Effector Memory CD8+T cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3389/fimmu.2017.00859 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Frontiers in Immunology, v.8 | - |
dc.identifier.wosid | 000406225300001 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.title | Frontiers in Immunology | - |
dc.citation.volume | 8 | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Soo | - |
dc.contributor.affiliatedAuthor | Kim, Kyung-Jin | - |
dc.contributor.affiliatedAuthor | Doh, Junsang | - |
dc.identifier.scopusid | 2-s2.0-85025628713 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CD8(+) T-CELLS | - |
dc.subject.keywordPlus | CA2+-ACTIVATED K+ CHANNEL | - |
dc.subject.keywordPlus | ION CHANNELS | - |
dc.subject.keywordPlus | AUTOIMMUNE-DISEASES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | LYMPHOCYTES | - |
dc.subject.keywordPlus | KCA3.1 | - |
dc.subject.keywordPlus | KV1.3 | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | STIMULATION | - |
dc.subject.keywordAuthor | human effector memory CD8+T cells | - |
dc.subject.keywordAuthor | interleukin-7Ralow effector memory CD8+T cells | - |
dc.subject.keywordAuthor | calcium-activated potassium channel KCa3.1 | - |
dc.subject.keywordAuthor | voltage-gated potassium channel Kv1.3 | - |
dc.subject.keywordAuthor | T-cell motility | - |
dc.subject.keywordAuthor | transendothelial migration | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
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