Overexpression of MicA induces production of OmpC-enriched outer membrane vesicles that protect against Salmonella challenge
SCIE
SCOPUS
- Title
- Overexpression of MicA induces production of OmpC-enriched outer membrane vesicles that protect against Salmonella challenge
- Authors
- Choi, Hyun-il; Kim, Moonjeong; Jeon, Jinseong; Han, Jin Kwan; Kim, Kwang-sun
- Date Issued
- 2017-08
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- Outer membrane vesicles (OMVs) derived from bacteria are promising candidates for subunit vaccines. Stresses that modulate the composition of outer membrane proteins (OMPs) are important for OMV synthesis. Small RNAs (sRNAs) expressed in response to stress regulate OMPs, although the mechanism underlying sRNA-mediated OMV biogenesis and its utility for developing vaccine platforms remains to be elucidated. Here, we characterized the role of a sRNA, MicA, which regulates OmpA, a major OMP involved in both production of OMVs and reactive immunity against Salmonella challenge. A Salmonella strain overexpressing MicA generated more OMVs than a control strain. In addition, OmpC was the major component of MicA-derived OMV proteins. MicA-derived OMVs induced Thl-and Th17-type immune responses in vitro and reduced Salmonella-mediated lethality in a mouse model. Thus, OmpA-regulatory sRNA-derived OMVs may facilitate production of Salmonella-protective vaccines. (C) 2017 Elsevier Inc. All rights reserved.
- Keywords
- BACTERIA-INDUCED LETHALITY; ESCHERICHIA-COLI; EXTRACELLULAR VESICLES; CELL RESPONSES; RNA; IMMUNITY; RELEASE; IDENTIFICATION; BIOGENESIS; INFECTION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50677
- DOI
- 10.1016/j.bbrc.2017.06.152
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 490, no. 3, page. 991 - 996, 2017-08
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.