DC Field | Value | Language |
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dc.contributor.author | Kim, Seong Gyeong | - |
dc.contributor.author | Jang, Sungho | - |
dc.contributor.author | Lim, Jae Hyung | - |
dc.contributor.author | Jeon, Byoung Seung | - |
dc.contributor.author | Kim, Jungyeon | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.contributor.author | Sang, Byoung-In | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2018-06-15T05:51:05Z | - |
dc.date.available | 2018-06-15T05:51:05Z | - |
dc.date.created | 2018-01-24 | - |
dc.date.issued | 2018-01 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/50911 | - |
dc.description.abstract | The aim of this study is to demonstrate that rebalancing of metabolic fluxes at acetyl-CoA branch node can substantially improve the titer and productivity of hexanoic acid in recombinant Escherichia coli strains. First, a hexanoic acid-producing E. coli strain was constructed by expressing genes encoding beta-ketothiolase (BktB) from Cupriavidus necator and acetyl-CoA transferase (ACT) from Megasphaera sp. MH in a butyric acid producer strain. Next, metabolic flux was optimized at the acetyl-CoA branch node by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four synthetic 5'- untranslated regions were designed for atoB using UTR Designer to modulate the expression level of the gene. Notably, the productivity of the optimized strain (14.7 mg/L/h) was the highest among recombinant E. coli strains in literature when using a similar inoculum size for fermentation. These results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.relation.isPartOf | BIORESOURCE TECHNOLOGY | - |
dc.subject | CHAIN LENGTH | - |
dc.subject | PATHWAY | - |
dc.subject | COENZYME | - |
dc.subject | GLUCOSE | - |
dc.title | Optimization of hexanoic acid production in recombinant Escherichia coli by precise flux rebalancing | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.biortech.2017.10.014 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.247, pp.1253 - 1257 | - |
dc.identifier.wosid | 000417841800158 | - |
dc.date.tcdate | 2018-03-23 | - |
dc.citation.endPage | 1257 | - |
dc.citation.startPage | 1253 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 247 | - |
dc.contributor.affiliatedAuthor | Kim, Seong Gyeong | - |
dc.contributor.affiliatedAuthor | Jung, Gyoo Yeol | - |
dc.identifier.scopusid | 2-s2.0-85031691701 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CHAIN LENGTH | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | COENZYME | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordAuthor | Hexanoic acid | - |
dc.subject.keywordAuthor | Flux rebalancing | - |
dc.subject.keywordAuthor | Acetyl-CoA acetyltransferase | - |
dc.subject.keywordAuthor | 5 &apos | - |
dc.subject.keywordAuthor | -UTR | - |
dc.subject.keywordAuthor | Acetyl-CoA transferase | - |
dc.relation.journalWebOfScienceCategory | Agricultural Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Energy & Fuels | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Energy & Fuels | - |
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