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Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules SCIE SCOPUS

Title
Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
Authors
Ahn, GeunseonMin, Kyung-HyunKim, ChanghwanLee, Jeong-SeokKang, DongguWon, Joo-YunCho, Dong-WooKim, Jun-YoungJin, SongwanYun, Won-SooShim, Jin-Hyung
Date Issued
2017-08
Publisher
Nature Publishing Group
Abstract
Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have drawbacks for cell printing, e.g. inappropriate crosslinking and liquid-like rheological properties, which hinder precise 3D shaping. Therefore, in this study, we investigated the influence of various factors (e.g. bioink concentration, viscosity, and extent of crosslinking) on cell printing and established a new 3D cell printing system equipped with heating modules for the precise stacking of decellularized extracellular matrix (dECM)-based 3D cell-laden constructs. Because the pH-adjusted bioink isolated from native tissue is safely gelled at 37 degrees C, our heating system facilitated the precise stacking of dECM bioinks by enabling simultaneous gelation during printing. We observed greater printability compared with that of a non-heating system. These results were confirmed by mechanical testing and 3D construct stacking analyses. We also confirmed that our heating system did not elicit negative effects, such as cell death, in the printed cells. Conclusively, these results hold promise for the application of 3D bioprinting to tissue engineering and drug development.
URI
https://oasis.postech.ac.kr/handle/2014.oak/50924
DOI
10.1038/s41598-017-09201-5
ISSN
2045-2322
Article Type
Article
Citation
Scientific Reports, vol. 7, 2017-08
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조동우CHO, DONG WOO
Dept of Mechanical Enginrg
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