Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
SCIE
SCOPUS
- Title
- Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
- Authors
- Kubo, Minoru; Nango, Eriko; Tono, Kensuke; Kimura, Tetsunari; Owada, Shigeki; Song, Changyong; Mafune, Fumitaka; Miyajima, Ken; Takeda, Yoshihiro; Kohno, Jun-ya; Miyauchi, Naoya; Nakane, Takanori; Tanaka, Tomoyuki; Nomura, Takashi; Davidsson, Jan; Tanaka, Rie; Murata, Michio; Kameshima, Takashi; Hatsui, Takaki; Joti, Yasumasa; Neutze, Richard; Yabashi, Makina; Iwata, So
- Date Issued
- 2017-09
- Publisher
- Blackwell Publishing Inc.
- Abstract
- X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/92029
- DOI
- 10.1107/S160057751701030X
- ISSN
- 0909-0495
- Article Type
- Article
- Citation
- Journal of Synchrotron Radiation, vol. 24, page. 1086 - 1091, 2017-09
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